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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb 4176 100 µl
Western Blotting Immunoprecipitation Immunofluorescence
H 92 Rabbit IgG
β-Catenin (D10A8) XP® Rabbit mAb 8480 100 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry Chromatin Immunoprecipitation
H M R Mk 92 Rabbit IgG

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

1.  Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.

2.  Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell Dev. Biol. 14, 59-88.

3.  Amit, S. et al. (2002) Genes Dev 16, 1066-76.

4.  Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.

5.  Liu, C. et al. (2002) Cell 108, 837-47.

6.  Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.

7.  Yost, C. et al. (1996) Genes Dev 10, 1443-54.

8.  Morin, P.J. et al. (1997) Science 275, 1787-90.

Entrez-Gene Id 1499
Swiss-Prot Acc. P35222

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.

PhosphoPlus® β-Catenin (Ser675) Antibody Duet