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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
α-E-Catenin (23B2) Rabbit mAb 3240 40 µl
Western Blotting Immunoprecipitation
H M Mk 100 Rabbit IgG
N-Cadherin (D4R1H) XP® Rabbit mAb 13116 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence
H 140 Rabbit IgG
E-Cadherin (24E10) Rabbit mAb 3195 40 µl
Western Blotting Immunohistochemistry Immunofluorescence Flow Cytometry
H M 135 Rabbit IgG
P-Cadherin (C13F9) Rabbit mAb 2189 40 µl
Western Blotting Immunofluorescence
H 120 Rabbit IgG
Pan-Cadherin (28E12) Rabbit mAb 4073 40 µl
Western Blotting
H M R 130-150 Rabbit IgG
β-Catenin (D10A8) XP® Rabbit mAb 8480 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry Chromatin Immunoprecipitation
H M R Mk 92 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting

Product Description

This Cadherin-Catenin Antibody Sampler kit contains reagents to examine the total protein levels of key proteins found in cell-cell adherens junctions. The kit contains enough primary and secondary antibodies to perform four Western blot experiments.

Specificity / Sensitivity

Each antibody in the Cadherin-Catenin Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Pan-Cadherin (28E12) Rabbit mAb #4073 detects endogenous levels of total cadherin proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human α-E-catenin, residues surrounding Arg526 of human N-cadherin protein, residues near the carboxy terminus of human P-cadherin, residues surrounding 780 of human E-cadherin, residues surrounding Pro714 of human ß-catenin protein, and a synthetic peptide corresponding to a conserved region of human N-, R-, E- and P-Cadherin.

Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of cadherins that are transmembrane proteins that bind cadherins on adjacent cells in a calcium dependent manner. On the cytoplasmic side of adherens junctions, the cadherins associate with β-catenin, γ-catenin and p120 catenin (δ). β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). Recent studies indicate that cancer cells exhibit increased N-cadherin and diminished E-cadherin expression. E-cadherin is considered a suppressor of invasive cancer cell growth and this change in cadherin expression associated with cancer progression is termed the “cadherin switch”. β-catenin is one of the key downstream effectors in the Wnt signaling pathway and has been implicated in early embryonic development and tumorigenesis (3-5).

1.  Wheelock, M.J. and Johnson, K.R. (2003) Annu Rev Cell Dev Biol 19, 207-35.

2.  Christofori, G. (2003) EMBO J 22, 2318-23.

3.  Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.

4.  Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell Dev. Biol. 14, 59-88.

5.  Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.

Entrez-Gene Id 1495 , 1499 , 999 , 1000 , 1001 , 1002
Swiss-Prot Acc. P35221 , P35222 , P12830 , P19022 , P22223 , P55283

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.

Cadherin-Catenin Antibody Sampler Kit