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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
MEK1/2 (D1A5) Rabbit mAb 8727 40 µl
Western Blotting Immunofluorescence Flow Cytometry
H M R Mk Dm 45 Rabbit IgG
AIF (D39D2) XP® Rabbit mAb 5318 40 µl
Western Blotting Immunoprecipitation Immunofluorescence
H M R Mk 67 Rabbit IgG
Histone H3 (D1H2) XP® Rabbit mAb 4499 40 µl
Western Blotting Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Mk 17 Rabbit IgG
Vimentin (D21H3) XP® Rabbit mAb 5741 40 µl
Western Blotting Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Mk 57 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting

Product Description

The Cell Fractionation Antibody Sampler Kit provides an economical means for determining the purity of each distinctly separated subcellular fraction by western blot using Cell Signaling Technology's Cell Fractionation Kit #9038. This antibody sampler kit includes enough primary antibody to perform at least four western blots per primary antibody.

Specificity / Sensitivity

Each antibody in the Cell Fractionation Antibody Sampler Kit recognizes endogenous levels of its respective target protein. The antibodies do not cross-react with other family members.

Each antibody has been validated using the Cell Fractionation Kit #9038. Expression of these proteins may vary in different cells and tissues.

Source / Purification

Rabbit monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala520 of human AIF protein, the carboxy terminus of human histone H3 protein, residues surrounding Ala220 of human MEK1 protein, and residues surrounding Arg45 of human vimentin protein.

Knowledge of the subcellular location of a protein may reveal the potential role it plays in a variety of cellular processes. Antibodies in the Cell Fractionation Antibody Sampler Kit can be used as a marker to ensure that each subcellular fraction is efficiently separated from the next. MEK1 and MEK2, also called MAPK or Erk Kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade (1). Apoptosis-inducing factor (AIF) is a ubiquitously expressed flavoprotein that plays a critical role in caspase-independent apoptosis (2). Core histone protein, H3 is one of the primary building blocks of chromatin that makes up the nucleosome (3). Vimentin is a cell-specific intermediate filament with mesenchyme origin that contributes to making up of the cytoskeleton (4). MEK1/2, AIF, histone H3, and vimentin localize to the cytoplasm, mitochondria, nucleus, and cytoskeleton, respectively.

1.  Crews, C.M. et al. (1992) Science 258, 478-480.

2.  Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.

3.  Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51.

4.  Lipton, S.A. and Bossy-Wetzel, E. (2002) Cell 111, 147-50.

Entrez-Gene Id 8350 , 5604 , 5605 , 9131 , 7431
Swiss-Prot Acc. P68431 , Q02750 , P36507 , O95831 , P08670

Protein Specific References

Zhong S et al. (2001) J Biol Chem 276, 33213–9

Li J et al. (2002) J Biol Chem 277, 49504–10

Sugiyama K et al. (2002) Oncogene 21, 3103–11

Li F et al. (2002) EMBO Rep 3, 767–73

Martens JH et al. (2003) Mol Cell Biol 23, 1808–16

Dai, J. et al. (2005) Genes Dev 19, 472-88.

Espino PS et al. (2006) Cancer Res 66, 4610–6

Park JH et al. (2006) Cancer Res 66, 9186–95

Soncini C et al. (2006) Clin Cancer Res 12, 4080–9

Idikio HA and (2006) Anticancer Res 26, 4687–94

Wang GG et al. (2007) Nat Cell Biol 9, 804–12

Duan Q et al. (2008) J Biol Chem 283, 33585–90

Dawson MA et al. (2009) Nature 461, 819–22

Cheng MF et al. (2009) Histol Histopathol 24, 1105–11

Hurd PJ et al. (2009) J Biol Chem 284, 16575–83

McGinty RK et al. (2009) ACS Chem Biol 4, 958–68

Banck MS et al. (2009) Epigenetics 4, 100–6

Wu Y et al. (2010) BMC Cancer 10, 32

Pasini D et al. (2010) Nucleic Acids Res 38, 4958–69

Sakabe K and Hart GW (2010) J Biol Chem 285, 34460–8

Gehani SS et al. (2010) Mol Cell 39, 886–900

Jung HR et al. (2010) Mol Cell Proteomics 9, 838–50

Davies GF et al. (2010) Cancer Lett 288, 236–50

Aguilera C et al. (2011) Nature 469, 231–5

Huertas D et al. (2012) Oncogene 31, 1408–18

Joosten M et al. (2013) Haematologica 98, 247–54

Sayegh J et al. (2013) J Biol Chem 288, 9408–17

Bondzi C et al. (2000) Oncogene 19, 5030–3

Wang R et al. (2007) Am J Physiol Lung Cell Mol Physiol 292, L240–8

Wang Z et al. (2007) Mol Cell Proteomics 6, 1365–79

Barberis L et al. (2009) Eur J Immunol 39, 1136–46

Tang DD et al. (2005) Biochem J 388, 773–83

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Fractionation Antibody Sampler Kit