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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb 5625 100 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H Mk 89 Rabbit IgG
PARP (46D11) Rabbit mAb 9532 100 µl
Western Blotting Immunoprecipitation Immunofluorescence
H M R Mk 116, 89 Rabbit 

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

1.  Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.

2.  Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.

3.  Nicholson, D.W. et al. (1995) Nature 376, 37-43.

4.  Cohen, G.M. (1997) Biochem. J. 326, 1-16.

5.  Tewari, M. et al. (1995) Cell 81, 801-809.

6.  Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.

Entrez-Gene Id 142
Swiss-Prot Acc. P09874

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.

PhosphoPlus® Cleaved PARP (Asp214) Antibody Duet