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H Mk Endogenous 80, 82 Rabbit

Western blot analysis of extracts from COS and SK-MEL-28 cell lines using CUL4A Antibody.

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Product Usage Information

Application Dilutions
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Cul4A Antibody detects endogenous levels of total human CUL4A protein.

Species Reactivity: Human, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residues surrounding Ser12 of human CUL4A. Antibodies are purified by peptide affinity chromatography.

Cullin-4 (CUL4) is a member of the cullin family of related ubiquitin ligases (1). A pair of mammalian CUL4 proteins - CUL4A and CUL4B - has been identified. The carboxy-terminal domain of CUL4 interacts with Rbx1 and E2 enzyme while the amino-terminal CUL4 domain interacts with BPB domain of UV-damaged DNA binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex (2). This CUL4-DDB1 complex binds a variety of WD40-containing proteins that help to enhance additional substrate recruitment (2,3). The CUL4 complex has been shown to target substrates involved in DNA repair, cell cycle progression (2), transcription (3), and development (2-6).

1.  Higa, L.A. and Zhang, H. (2007) Cell Div 2, 5.

2.  Ohtake, F. et al. (2007) Nature 446, 562-6.

3.  Shiyanov, P. et al. (1999) J Biol Chem 274, 35309-12.

4.  Petroski, M.D. and Deshaies, R.J. (2005) Nat Rev Mol Cell Biol 6, 9-20.

5.  Lee, J. and Zhou, P. (2007) Mol Cell 26, 775-80.

6.  Ghosh, P. et al. (2008) Cell Cycle 7, 373-81.

Entrez-Gene Id 8451
Swiss-Prot Acc. Q13619

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

CUL4A Antibody