REACTIVITY | H |
SENSITIVITY | Endogenous |
MW (kDa) | 142 |
Source/Isotype | Mouse IgG1 |
Product Information
Application | Dilution |
---|---|
Immunofluorescence (Immunocytochemistry) | 1:100 |
Achieve higher quality immunofluorescent images using the efficient and cost-effective, pre-made reagents in our #12727 Immunofluorescence Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Recommended Fluorochrome-conjugated Anti-Mouse secondary antibodies:
NOTE: Cells should be grown, treated, fixed and stained directly in multi-well plates, chamber slides or on coverslips.
Aspirate liquid, then cover cells to a depth of 2–3 mm with 4% formaldehyde diluted in 1X PBS.
NOTE: Formaldehyde is toxic, use only in a fume hood.
NOTE: All subsequent incubations should be carried out at room temperature unless otherwise noted in a humid light-tight box or covered dish/plate to prevent drying and fluorochrome fading.
posted November 2006
revised November 2013
Protocol Id: 148
Human
Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the amino terminus of human DSG2 protein.
Desmosomes are a class of intracellular junction that tightly link adjacent cells in mechanically stressed tissues such as the epithelium and myocardium (1). They derive their characteristic strength from the protein desmoplakin, which acts as a tether by binding the cytoplasmic component of the desmosome at its N terminus (2), while its C terminus is anchored to the intermediate-filament cytoskeleton (3). Desmogleins and desmocollins belong to the superfamily of cadherin proteins, the "glue" of the desmosome, as they are essential for strong cell-cell contacts (4). There are 4 types of desmogleins in humans, DSG1-4, and 3 types of desmocollins, DSC1-3. DSG2 is expressed in all desmosome bearing tissues, while other desmosome cadherin proteins have more specialized tissue expression. Research studies have shown that aberrant expression due to mutation of DSG2 is associated with arrhythmogenic right ventricular cardiomyopathy (5,6). Research has also shown that mutation and altered expression of DSG1 and 3 have been associated with autoimmune disorders such as Pemphigus, inherited disorders such as defective hair-follicle differentiation, and striate palmoplantar keratoderma, an epidermal-thickening disease (reviewed in 7).
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