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H M R Endogenous 135 Rabbit

Western blot analysis of extracts from MCF-7, NIH/3T3 and PC12 cells using HAUSP Antibody.

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Product Usage Information

Application Dilutions
Western Blotting 1:1000

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

HAUSP Antibody detects endogenous levels of total HAUSP protein.

Species Reactivity: Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp438 of human HAUSP. Antibodies are purified by peptide affinity chromatography.

Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action (1,2). Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD and JAMM enzymes. Herpesvirus-associated ubiquitin-specific protease (HAUSP, USP7) is an important deubiquitinase belonging to USP subfamily. A key HAUSP function is to bind and deubiquitinate the p53 transcription factor and an associated regulator protein Mdm2, thereby stabilizing both proteins (3,4). In addition to regulating essential components of the p53 pathway, HAUSP also modifies other ubiquitinylated proteins such as members of the FoxO family of forkhead transcription factors and the mitotic stress checkpoint protein CHFR (5,6).

1.  Nijman, S.M. et al. (2005) Cell 123, 773-86.

2.  Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613.

3.  Li, M. et al. (2002) Nature 416, 648-53.

4.  Brooks, C.L. et al. (2007) Oncogene 26, 7262-6.

5.  van der Horst, A. et al. (2006) Nat Cell Biol 8, 1064-73.

6.  Oh, Y.M. et al. (2007) Biochem Biophys Res Commun 357, 615-9.

Entrez-Gene Id 7874
Swiss-Prot Acc. Q93009

For Research Use Only. Not For Use In Diagnostic Procedures.
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HAUSP Antibody