Cat. # | Size | Qty. | Price |
---|---|---|---|
99756S | 100 µl |
|
REACTIVITY | H |
SENSITIVITY | Endogenous |
MW (kDa) | 50, 65 |
Source/Isotype | Rabbit IgG |
Product Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 263
Human
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu466 of human HPSE protein.
Heparanase (HPSE) is an endo-β-D-endoglycosidase that catalyzes the cleavage heparan sulfate (HS) side chains from proteoglycans at the cell surface (1). The protein is synthesized as a 65 kDa inactive precursor that undergoes proteolytic processing, yielding 8 kDa and 50 kDa protein subunits that heterodimerize to form an active enzyme (2). HPSE serves as a key modulator of extracellular matrix (ECM) basic structure. The cleavage of HS from proteoglycans and associated changes at the ECM also cause the release of growth factors from the matrix to bind to their cognate receptors, leading to activation of downstream signaling pathways (3). A small percentage of HPSE localizes to specialized lipid raft regions. There, HPSE activates multiple signaling pathways, such as Akt, ERK, and Src pathways, by a nonenzymatic mechanism mediated by its C-terminal domain (4,5). HPSE is involved in the development of diseases such as fibrosis initiation, inflammation, tumor progression, and viral infection (6-9). HPSE inhibitors have been developed and pursued as novel therapeutics for these diseases (10-12).
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