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H M R Mk Endogenous 100 Rabbit IgG

Western blot analysis of extracts from various cell types using MCM3 (D47B6) Rabbit mAb.

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Product Usage Information

Application Dilutions
Western Blotting 1:1000

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

MCM3 (D47B6) Rabbit mAb detects endogenous levels of total MCM3 protein.

Species Reactivity: Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues of human MCM3.

The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork (1-5). This complex is a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication (reviewed in 2). Licensing of the chromatin permits the DNA to replicate only once per cell cycle, thereby helping to ensure that genetic alterations and malignant cell growth do not occur (reviewed in 3). Phosphorylation of the MCM2, MCM3, MCM4, and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis (6-8). CDK1 phosphorylation of MCM3 at Ser112 during late mitosis/early G1 phase has been shown to initiate complex formation and chromatin loading in vitro (8). Phosphorylation of MCM2 at serine 139 by cdc7/dbf4 coincides with the initiation of DNA replication (9). MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Studies have shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest (reviewed in 3,10).

1.  Lei, M. and Tye, B.K. (2001) J Cell Sci 114, 1447-54.

2.  Lygerou, Z. and Nurse, P. (2000) Science 290, 2271-3.

3.  Forsburg, S.L. (2004) Microbiol Mol Biol Rev 68, 109-31.

4.  Tye, B.K. and Sawyer, S. (2000) J Biol Chem 275, 34833-6.

5.  Tsuji, T. et al. (2006) Mol Biol Cell 17, 4459-72.

6.  Labib, K. et al. (2000) Science 288, 1643-7.

7.  Charych, D.H. et al. (2008) J Cell Biochem 104, 1075-86.

8.  Masai, H. et al. (2006) J Biol Chem 281, 39249-61.

9.  Lin, D.I. et al. (2008) Proc Natl Acad Sci U S A 105, 8079-84.

10.  Bailis, J.M. et al. (2008) Mol Cell Biol 28, 1724-38.

Entrez-Gene Id 4172
Swiss-Prot Acc. P25205

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

MCM3 (D47B6) Rabbit mAb