Cat. # | Size | Qty. | Price |
---|---|---|---|
71789S | 100 µl |
|
REACTIVITY | H |
SENSITIVITY | Endogenous |
MW (kDa) | 20-30 |
Source/Isotype | Rabbit IgG |
Product Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
Human
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human PGLYRP-1/PGRP-S protein.
The human peptidoglycan recognition protein (PGRP) family is composed of four independent genes named for the relative length of their translated product. Human PGRP family proteins are soluble pattern-recognition receptors of the innate immune response that bind peptidoglycan and Gram-positive bacteria, and have direct bactericidal activity (1,2). PGRP-short (PGRP-S), also known as PGLYRP-1 and Tag7, is 196 amino acids (aa) in length (3). PGLYRP-1/PGRP-S is a secreted glycoprotein expressed by neutrophils that participates in killing phagocytized bacteria (4,5). PGLYRP-1/PGRP-S also has been shown to form a stable cytotoxic complex with the heat shock protein Hsp70 to induce tumor cell death (6,7). PGRP-long (PGRP-L), also known as PGLYRP-2, is 576 aa long. PGLYRP-2/PGRP-L is an N-acetylmuramoyl-L-alanine amidase that hydrolyzes bacterial peptidoglycan (8,9). PGRP-intermediate alpha, also known as PGLYRP-3, and PGRP-intermediate beta, also known as PGLYRP-4, are 341 aa and 373 aa long, respectively. PGLYRP-3 and PGLYRP-4 are co-expressed in the same cells and are exclusively secreted as homo- or heterodimers with antibacterial function (10).
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