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H Endogenous 50 Rabbit

Western blot analysis of extracts from OVCAR8, SNB19 and Wek293 cells using PHD-2/Egln1 Antibody.

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Product Usage Information

Application Dilutions
Western Blotting 1:1000

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

PHD-2/Egln1 Antibody detects endogenous levels of total PHD-2/Egln1 protein.

Species Reactivity: Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val226 of human PHD-2/Egln1 protein. Antibodies are purified by peptide affinity chromatography.

PHD1 (Egln2), PHD-2 (Egln1), and PHD3 (Egln3) are members of the Egln family of proline hydroxylases. They function as oxygen sensors that catalyze the hydroxylation of HIF on prolines 564 and 402, initiating the first step of HIF degradation through the VHL/ubiquitin pathway (1,2). PHD1 is highly expressed in a wide array of tissues whereas PHD2 and PHD3 are expressed mainly in heart and skeletal muscle (1,3). The mRNA levels of PHD are upregulated by HIF through the hypoxia-response element under low oxygen conditions (4-7). These three enzymes also exhibit different peptide specificity target proteins, PHD1 and PHD2 can hydroxylate both proline 402 and proline 564, but PHD3 can only hydroxylate proline 564 (2,8). In addition to HIF, PHD enzymes have also has been shown to catalyze the hydroxylation of RNA polymerase subunits and myogenin (3,9).

1.  Freeman, R.S. et al. (2003) Mol Cells 16, 1-12.

2.  Villar, D. et al. (2007) Biochem J 408, 231-40.

3.  Fu, J. et al. (2007) J Biol Chem 282, 12410-8.

4.  D'Angelo, G. et al. (2003) J Biol Chem 278, 38183-7.

5.  del Peso, L. et al. (2003) J Biol Chem 278, 48690-5.

6.  Pescador, N. et al. (2005) Biochem J 390, 189-97.

7.  Metzen, E. et al. (2005) Biochem J 387, 711-7.

8.  Hirsilä, M. et al. (2003) J Biol Chem 278, 30772-80.

9.  Mikhaylova, O. et al. (2008) Mol Cell Biol 28, 2701-17.

Entrez-Gene Id 54583
Swiss-Prot Acc. Q9GZT9

For Research Use Only. Not For Use In Diagnostic Procedures.
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PHD-2/Egln1 Antibody