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H Endogenous 80 Rabbit

Western blot analysis of extracts from HT-1376 cells, untreated or pervanadate-treated ( 1 mM, 20 minutes), using Phospho-p73 (Tyr99) Antibody.

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Product Usage Information

Application Dilutions
Western Blotting 1:1000

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-p73 (Tyr99) Antibody detects endogenous levels of p73 only when phosphorylated at tyrosine 99. This antibody cross-reacts with p63 when phosphorylated at tyrosine 149.

Species Reactivity: Human
Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr99 of human p73. Antibodies are purified by protein A and peptide affinity chromatography.

The p53 family member, p73, exists in multiple isoforms/splice variants and can induce apoptosis and cell cycle arrest in response to DNA damage via its activity as a transcription regulator (1-3). Upon DNA damage, p73 is phosphorylated at Tyr99 by c-Abl, causing translocation to the nuclear matrix (4). DNA damage-induced acetylation of p73 at Lys321 by the acetyltransferase p300 has also been reported to enhance transcription of genes including that of p53AIP1 (5). Another report, however, indicates that p300 does not acetylate full-length p73 in vivo (6).

While the sequences surrounding p73 Tyr99 and p63 Tyr149 are very similar, only p73 co-localizes with c-Abl following gamma irradiation, suggesting that p63 is not a c-Abl substrate (7).

1.  Kaghad, M. et al. (1997) Cell 90, 809-19.

2.  Jost, C.A. et al. (1997) Nature 389, 191-4.

3.  De Laurenzi, V.D. et al. (1999) Cell Death Differ 6, 389-90.

4.  Ben-Yehoyada, M. et al. (2003) J Biol Chem 278, 34475-82.

5.  Costanzo, A. et al. (2002) Mol Cell 9, 175-86.

6.  Zeng, X. et al. (2001) J Biol Chem 276, 48-52.

7.  Hamer, G. et al. (2001) Oncogene 20, 4298-4304.

Entrez-Gene Id 7161
Swiss-Prot Acc. O15350

For Research Use Only. Not For Use In Diagnostic Procedures.
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Phospho-p73 (Tyr99) Antibody