Cat. # | Size | Qty. | Price |
---|---|---|---|
15641S | 100 µl |
|
REACTIVITY | H M |
SENSITIVITY | Endogenous |
MW (kDa) | |
Source/Isotype | Rabbit IgG |
Product Information
Application | Dilution |
---|---|
Immunohistochemistry (Paraffin) | 1:500 - 1:2000 |
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: Do not allow slides to dry at any time during this procedure.
For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; follow with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.
RECOMMENDED DETECTION REAGENTS |
SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) #8114 | SignalStain® Boost IHC Detection Reagent (AP, Rabbit) #18653 |
---|---|---|
COMPATIBLE CHROMOGEN |
SignalStain® DAB Substrate Kit #8059 | SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit #76713 |
SignalStain® Vivid Purple Peroxidase Substrate Kit #96632 | SignalStain® Ultra Blue Alkaline Phosphatase Substrate Kit #12824 | |
SignalStain® Deep Black Peroxidase Substrate Kit #72986 | ||
SignalStain® Radiant Yellow Peroxidase Substrate Kit #69644 |
NOTE: Use of detection reagents other than those specified in this protocol may require further optimization of the primary antibody to account for the different sensitivities of the detection reagents.
posted February 2010
revised April 2020
Protocol Id: 1989
Human, Mouse
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human PTPRZ1 protein.
PTPRZ1, also known as protein tyrosine phosphatase receptor zeta, is a highly glycosylated membrane protein that is expressed abundantly in normal brain, in a spatially restricted pattern. The functions of PTPRZ1 in neural tissue are thought to be diverse, including development, neural development, neural myelination, and memory formation (1). PTPRZ1 can be found in 2 forms, a membrane-associated glycosylated form and a non-glycosylated, secreted form. In patients with glioma, the soluble form is found to be unregulated in cerebrospinal fluid (2). It is for this and other reports, that PTPRZ1 is a target of pharmaceutical interest. PTPRZ1 has also been reported to be upregulated. Interestingly, increased expression of both membrane bound and soluble PTPRZ1 has also been found in various cancer types, including breast, lung, cervical, hepatocellular carcinoma, and renal tumors (3). In breast cancer, the distribution between localizations of membrane and soluble forms has been reported to change with cancer development, progression, and metastasis.
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