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To Purchase # 9969S

9969S 1 Kit (4 x 40 µl) $329.00
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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-Rb (Ser780) (D59B7) Rabbit mAb 8180 40 µl
Western Blotting Immunoprecipitation
H M R Mk 110 Rabbit IgG
Phospho-Rb (Ser795) Antibody 9301 40 µl
Western Blotting Immunoprecipitation
H R Mk 110 Rabbit 
Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb 8516 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Mk 110 Rabbit IgG
Rb (4H1) Mouse mAb 9309 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry Chromatin Immunoprecipitation
H Mk B Pg 110 Mouse IgG2a
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
Western Blotting
All Horse 

Product Description

The Rb Antibody Sampler Kit provides reagents and protocols to investigate cell cycle progression within cells. The kit contains primary and secondary antibodies to perform four Western blot experiments with each antibody.


Specificity / Sensitivity

All antibodies contained in this kit detect endogenous levels of their respective target protein.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide and are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with recombinant human proteins or synthetic peptides.

The retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).


1.  Sherr, C.J. (1996) Science 274, 1672-7.

2.  Nevins, J.R. (1992) Science 258, 424-9.

3.  Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-90.

4.  Hu, Q.J. et al. (1990) EMBO J 9, 1147-55.

5.  Knudsen, E.S. and Wang, J.Y. (1997) Mol Cell Biol 17, 5771-83.

6.  Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61.

7.  Connell-Crowley, L. et al. (1997) Mol Biol Cell 8, 287-301.

8.  Kitagawa, M. et al. (1996) EMBO J 15, 7060-9.

9.  Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9.


Entrez-Gene Id 5925
Swiss-Prot Acc. P06400

Protein Specific References

Brantley MA Jr and Harbour JW (2000) Cancer Res 60, 4320–3

Panigone S et al. (2000) Oncogene 19, 4035–41

Chan HM et al. (2001) Nat Cell Biol 3, 667–74

Taneja SS et al. (2001) J Cell Biochem 84, 188–99

Schroeder MD et al. (2002) Mol Endocrinol 16, 45–57

Balasenthil S and Vadlamudi RK (2003) J Biol Chem 278, 22119–27

Huang H et al. (2004) Oncogene 23, 2161–76

Benzeno S et al. (2004) Cancer Res 64, 3885–91

Youn CK et al. (2005) Nat Cell Biol 7, 137–47

Schmitz NM et al. (2005) Leukemia 19, 1783–7

Leibundgut K et al. (2005) Stem Cells 23, 1002–11

Wallick CJ et al. (2005) Oncogene 24, 5606–18

Roesch A et al. (2005) Mod Pathol 18, 565–72

Swarbrick A et al. (2005) Oncogene 24, 381–9

Ledl A et al. (2005) Oncogene 24, 3810–8

Hamdane M et al. (2005) J Cell Sci 118, 1291–8

Schmitz NM et al. (2006) Am J Pathol 169, 1074–9

Inoue Y et al. (2007) EMBO J 26, 2083–93

Park Y et al. (2008) Cell Cycle 7, 2384–91

Chang TW et al. (2008) Oncogene 27, 332–8

Boulay PL et al. (2011) Oncogene 30, 3846–61

Sen S et al. (2011) J Biol Chem 286, 36580–91

Lentine B et al. (2012) Cell Cycle 11, 3324–30

Brantley MA Jr and Harbour JW (2000) Cancer Res 60, 4320–3

Panigone S et al. (2000) Oncogene 19, 4035–41

Chan HM et al. (2001) Nat Cell Biol 3, 667–74

Taneja SS et al. (2001) J Cell Biochem 84, 188–99

Schroeder MD et al. (2002) Mol Endocrinol 16, 45–57

Balasenthil S and Vadlamudi RK (2003) J Biol Chem 278, 22119–27

Huang H et al. (2004) Oncogene 23, 2161–76

Benzeno S et al. (2004) Cancer Res 64, 3885–91

Youn CK et al. (2005) Nat Cell Biol 7, 137–47

Schmitz NM et al. (2005) Leukemia 19, 1783–7

Leibundgut K et al. (2005) Stem Cells 23, 1002–11

Wallick CJ et al. (2005) Oncogene 24, 5606–18

Roesch A et al. (2005) Mod Pathol 18, 565–72

Swarbrick A et al. (2005) Oncogene 24, 381–9

Ledl A et al. (2005) Oncogene 24, 3810–8

Hamdane M et al. (2005) J Cell Sci 118, 1291–8

Schmitz NM et al. (2006) Am J Pathol 169, 1074–9

Inoue Y et al. (2007) EMBO J 26, 2083–93

Park Y et al. (2008) Cell Cycle 7, 2384–91

Chang TW et al. (2008) Oncogene 27, 332–8

Boulay PL et al. (2011) Oncogene 30, 3846–61

Sen S et al. (2011) J Biol Chem 286, 36580–91

Lentine B et al. (2012) Cell Cycle 11, 3324–30

Brantley MA Jr and Harbour JW (2000) Cancer Res 60, 4320–3

Panigone S et al. (2000) Oncogene 19, 4035–41

Chan HM et al. (2001) Nat Cell Biol 3, 667–74

Taneja SS et al. (2001) J Cell Biochem 84, 188–99

Schroeder MD et al. (2002) Mol Endocrinol 16, 45–57

Balasenthil S and Vadlamudi RK (2003) J Biol Chem 278, 22119–27

Huang H et al. (2004) Oncogene 23, 2161–76

Benzeno S et al. (2004) Cancer Res 64, 3885–91

Youn CK et al. (2005) Nat Cell Biol 7, 137–47

Schmitz NM et al. (2005) Leukemia 19, 1783–7

Leibundgut K et al. (2005) Stem Cells 23, 1002–11

Wallick CJ et al. (2005) Oncogene 24, 5606–18

Roesch A et al. (2005) Mod Pathol 18, 565–72

Swarbrick A et al. (2005) Oncogene 24, 381–9

Ledl A et al. (2005) Oncogene 24, 3810–8

Hamdane M et al. (2005) J Cell Sci 118, 1291–8

Schmitz NM et al. (2006) Am J Pathol 169, 1074–9

Inoue Y et al. (2007) EMBO J 26, 2083–93

Park Y et al. (2008) Cell Cycle 7, 2384–91

Chang TW et al. (2008) Oncogene 27, 332–8

Boulay PL et al. (2011) Oncogene 30, 3846–61

Sen S et al. (2011) J Biol Chem 286, 36580–91

Lentine B et al. (2012) Cell Cycle 11, 3324–30


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

9969
Rb Antibody Sampler Kit