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REACTIVITY SENSITIVITY MW (kDa) SOURCE
H M R Hm Mk Z B Sc Endogenous 46, 54 Rabbit

Product Usage Information

Application Dilutions
Western Blotting 1:1000

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

SAPK/JNK Antibody detects endogenous levels of total SAPK/JNK protein.


Species Reactivity: Human, Mouse, Rat, Hamster, Monkey, Zebrafish, Bovine, S. cerevisiae

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a recombinant human JNK2 fusion protein. Antibodies are purified by protein A and peptide affinity chromatography.

The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing, resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5).


1.  Ichijo, H. (1999) Oncogene 18, 6087-93.

2.  Kyriakis, J.M. and Avruch, J. (2001) Physiol Rev 81, 807-69.

3.  Kyriakis, J.M. (1999) J Biol Chem 274, 5259-62.

4.  Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem Sci 23, 481-5.

5.  Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.

6.  Davis, R.J. (1999) Biochem Soc Symp 64, 1-12.


Entrez-Gene Id 5599
Swiss-Prot Acc. P45983


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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SAPK/JNK Antibody