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M R Endogenous 25 Rabbit

Western blot analysis of extracts from mouse and rat brain using SNAP25 (A195) Antibody.

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Product Usage Information

Application Dilutions
Western Blotting 1:1000

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

SNAP25 (A195) Antibody detects endogenous levels of total SNAP25 protein.

Species Reactivity: Mouse, Rat
Species predicted to react based on 100% sequence homology: Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala195 of human SNAP25. Antibodies are purified by protein A and peptide affinity chromatography.

The 25 kDa synaptosome-associated protein (SNAP25) is a target membrane soluble, N-ethylmaleimide-sensitive factor attachment protein receptor (t-SNARE) that is found on neuronal presynaptic membranes. SNAP25 forms a core complex with the SNARE proteins syntaxin and synaptobrevin to mediate synaptic vesicle fusion with the plasma membrane during Ca2+-dependent exocytosis (1). This complex is responsible for exocytosis of the neurotransmitter γ-aminobutyric acid (GABA). Neurotransmitter release is inhibited by proteolysis of SNAP25 by botulinum toxins A and E (2). SNAP25 plays a secondary role as a Q-SNARE involved in endosome fusion; the protein is associated with genetic susceptibility to attention-deficit hyperactivity disorder (ADHD) (3).

1.  Fan, H.P. et al. (2006) J Biol Chem 281, 28174-84.

2.  Salaün, C. et al. (2004) Biochim Biophys Acta 1693, 81-9.

3.  Aikawa, Y. et al. (2006) Mol Biol Cell 17, 2113-24.

Entrez-Gene Id 6616
Swiss-Prot Acc. P60880

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SNAP25 (A195) Antibody