Cat. # | Size | Qty. | Price |
---|---|---|---|
33942S | 100 µl |
|
REACTIVITY | H M R |
SENSITIVITY | Endogenous |
MW (kDa) | 70, 74 |
Source/Isotype | Rabbit IgG |
Product Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
Human, Mouse, Rat
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp536 of human SWAP70 protein.
SWAP70 is a Rac family guanine nucleotide exchange factor (GEF) (1). It is highly expressed by activated B cells. Following B cell stimulation, SWAP70 has been observed to translocate from the cytoplasm to the nucleus, where it plays a role in class switching, as well as to the membrane, where it associates with the B cell receptor (2,3). SWAP70 also plays a role in migration of B cells and other immune cell types including dendritic cells, eosinophils, and mast cells (4-7). Mice deficient in both SWAP70 and a related protein, DEF6, develop lupus-like autoimmunity due to misregulation of IRF4 in B cells and T cells leading to increased IL21 production and responsiveness (8).
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