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M Transfected Only Rabbit

Western blot analysis of extracts from COS cells, untransfected or transfected with mouse USP28, using USP28 Antibody.

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Product Usage Information

Application Dilutions
Western Blotting 1:1000

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

USP28 Antibody detects transfected levels of USP28 protein.

Species Reactivity: Mouse
Species predicted to react based on 100% sequence homology: Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala11 of human USP28. Antibodies are purified by peptide affinity chromatography.

Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzymes (DUB) action (1,2). There are five DUB subfamilies including the USP, UCH, OTU, MJD and JAMM enzymes. USP28 is an important member of the USP subfamily (3). USP28 can bind to and deubiquinate several target proteins in DNA-damage pathway, resulting in their stability, including p53BP1 and Chk2 (4). USP28 also plays an important role in Myc related signaling by binding through FBW7α to Myc . It catalyzes the deubiquitinylation of Myc, thereby promoting its stabilization and contributing to tumor-cell growth (5).

1.  Nijman, S.M. et al. (2005) Cell 123, 773-86.

2.  Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613.

3.  Valero, R. et al. (2001) Genome Biol 2, RESEARCH0043.

4.  Zhang, D. et al. (2006) Cell 126, 529-42.

5.  Popov, N. et al. (2007) Nat Cell Biol 9, 765-74.

Entrez-Gene Id 57646
Swiss-Prot Acc. Q96RU2

Product Specific References

Mannava, S. et al. (2011) Oncogene , .

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

USP28 Antibody