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PTMScan® Motif Antibody Kits
New Format-Same Price

Now 10 Assays Per Kit

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Pricing & Additional Information

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Product Includes Cap Color Volume (with Count)
PTMScan® T*PP Motif (T*PP) Immunoaffinity Beads Blue 10 x 80 µl
PTMScan® IAP Buffer (10X) 9993 White 10 x 600 µl
PTMScan® Limited Use License  

Product Usage Information

Cells are lysed in a urea-containing buffer, cellular proteins are digested by proteases, and the resulting peptides are purified by reversed-phase, solid-phase extraction. Peptides are then subjected to immunoaffinity purification using a PTMScan® antibody conjugated to protein A agarose beads. Unbound peptides are removed through washing, and the captured PTM-containing peptides are eluted with dilute acid. Reversed-phase purification is performed on microtips to desalt and separate peptides from antibody prior to concentrating the enriched peptides for LC-MS/MS analysis. CST recommends the use of PTMScan® IAP Buffer #9993 included in the kit. An alternate PTMScan® IAP Buffer Plus Detergent #9992 which may reduce nonspecific interactions is available separately. A detailed protocol and Limited Use License allowing the use of the patented PTMScan® method is included with the kit.


Storage: Antibody beads supplied in IAP buffer containing 50% glycerol. Store at -20°C. Do not aliquot the antibody.

Product Description

PTMScan® Technology employs a proprietary methodology from Cell Signaling Technology (CST) for peptide enrichment by immunoprecipitation using a specific bead-conjugated antibody in conjunction with liquid chromatography (LC) tandem mass spectrometry (MS/MS) for quantitative profiling of post-translational modification (PTM) sites in cellular proteins. These include phosphorylation (PhosphoScan®), ubiquitination (UbiScan®), acetylation (AcetylScan®), and methylation (MethylScan®), among others. PTMScan® Technology enables researchers to isolate, identify, and quantitate large numbers of post-translationally modified cellular peptides with a high degree of specificity and sensitivity, providing a global overview of PTMs in cell and tissue samples without preconceived biases about where these modified sites occur (1). For more information on PTMScan® Proteomics Services, please visit www.cellsignal.com/common/content/content.jsp?id=ptmscan-services.


A T*PP phosphorylation motif has been extracted from a large-scale proteomic data set on HeLa nuclei (2). Western blot and proteomic analysis shows that phosphorylation on this motif is sensitive to cell cycle control. Similar motifs are phosphorylated by MAPK and CDK families of serine/threonine protein kinases, which play important roles in proliferation and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (motifs PX(S/T)P and (S/T)PXK/R) (3-5). The T*PP Motif (T*PP) XP® Rabbit mAb contained in this kit, which binds to the motif T*PP, was developed by CST for use in the study and discovery of new kinase substrates.

In this assay, a PTMScan® (T*PP) Motif Antibody bead conjugates are used to specifically enrich phosphopeptides containing the T*PP motif (T* = phospho-threonine).


1.  Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.

2.  Schwartz, D. and Gygi, S.P. (2005) Nat Biotechnol 23, 1391-8.

3.  Seger, R. and Krebs, E.G. (1995) FASEB J 9, 726-35.

4.  Cross, T.G. et al. (2000) Exp Cell Res 256, 34-41.

5.  Reynolds, C.H. et al. (2000) J Neurochem 74, 1587-95.



For Research Use Only. Not For Use In Diagnostic Procedures.
AcetylScan® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
MethylScan® is a trademark of Cell Signaling Technology, Inc.
PTMScan® is a trademark of Cell Signaling Technology, Inc.
UbiScan® is a trademark of Cell Signaling Technology, Inc.
XP® is a trademark of Cell Signaling Technology, Inc.
Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.