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8887S 250 µl

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Flow Cytometry

Flow cytometric analysis of untreated Jurkat cells using Akt (5G3) Mouse mAb #2966 detected with Anti-mouse IgG (H+L), F(ab')2 Fragment (PE Conjugate) (blue) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (red).

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Product Usage Information

The optimal dilution of the secondary antibody should be determined for each application. However, a final dilution of 1:500 should yield acceptable results for most flow cytometry applications.


Storage: Store at 4°C.

Product Description

Anti-mouse IgG (H+L), F(ab')2 Fragment was conjugated to phycoerythrin (PE) under optimal conditions and formulated at 1 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.


Specificity / Sensitivity

F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against human IgG and human serum.


This product has been optimized for use as a secondary antibody in FLOW ctytometry. Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.



For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.