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To Purchase # 13803S

13803S 300 µl (3 nmol) $249.00
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Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® VIMP siRNA I (+), or SignalSilence® VIMP siRNA II #13818 (+), using VIMP Antibody #13738 (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The VIMP Antibody confirms silencing of VIMP expression, while the GAPDH (D16H11) XP® Rabbit mAb is used as a loading control.

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Product Usage Information

CST recommends transfection with 100 nM SignalSilence® VIMP siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow the protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.


Storage: SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.

Product Description

SignalSilence® VIMP siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit VIMP expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.


Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

VCP-interacting membrane protein (VIMP, selenoprotein S) is a putative reductase and endoplasmic reticulum (ER)-resident protein involved in the ER-associated degradation (ERAD) pathway (1,2). Research studies indicate that VIMP may play a protective role against inflammation and reduce ER-stress (3). The VIMP protein is a single-pass, transmembrane protein that recruits the cytosolic p97/VCP AAA-ATPase and its cofactors, UFD1 and NPL4, to the ER membrane (4). An ER membrane complex containing Derlin-1 and VIMP forms a critical node in the ERAD machinery and links substrate recognition in the ER lumen with the retrotranslocation function of the p97/VCP AAA-ATPase in the cytosol (1,4). Polymorphisms in the corresponding VIMP gene are associated with spontaneous preterm births and cardiovascular disease risk (5,6) while other studies do not support a correspondence between VIMP polymorphisms and inflammatory disorders (7).


1.  Lilley, B.N. and Ploegh, H.L. (2005) Proc Natl Acad Sci U S A 102, 14296-301.

2.  Christensen, L.C. et al. (2012) J Biol Chem 287, 26388-99.

3.  Fradejas, N. et al. (2011) Glia 59, 959-72.

4.  Ye, Y. et al. (2004) Nature 429, 841-7.

5.  Wang, Y. et al. (2013) PLoS One 8, e65657.

6.  Cox, A.J. et al. (2013) Acta Diabetol 50, 391-9.

7.  Martínez, A. et al. (2008) BMC Genomics 9, 329.


Entrez-Gene Id 55829
Swiss-Prot Acc. Q9BQE4


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
SignalSilence® is a trademark of Cell Signaling Technology, Inc.
XP® is a trademark of Cell Signaling Technology, Inc.