Upstream / Downstream

Explore pathways related to this product.

Important Ordering Details

Custom Ordering Details: Product is assembled upon order. Please allow up to three business days for your product to be processed.

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

To Purchase # 14100S

14100S 300 µl (3 nmol) $249.00
$ 0. 00

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

REACTIVITY
H

Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® APC11 siRNA I (+), using APC11 (D1E7Q) Rabbit mAb #14090 (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The APC11 (D1E7Q) Rabbit mAb confirms silencing of APC11, while the GAPDH (D16H11) XP® Rabbit mAb is used as a loading control.

Learn more about how we get our images
Image

Product Usage Information

CST recommends transfection with 100 nM SignalSilence® APC11 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.


Storage: APC11 siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.

Product Description

SignalSilence® APC11 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit APC11 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.


Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Cell proliferation in all eukaryotic cells depends strictly upon the ubiquitin ligase (E3) activity of the anaphase promoting complex/cyclosome (APC/C), whose main function is to trigger the transition of the cell cycle from metaphase to anaphase. APC/C performs its various functions by promoting the assembly of polyubiquitin chains on substrate proteins, which targets these proteins for degradation by the 26S proteasome (1,2). In humans, twelve different APC/C subunits have been identified. Like all E3 enzymes, APC/C utilizes ubiquitin residues that have been activated by E1 enzymes and then transferred to E2 enzymes. Indeed, APC/C has been shown to interact with UBE2S and UBE2C E2 enzymes, in part, via the RING-finger domain-containing subunit, APC11 (3-5). APC/C activity is also strictly dependent upon its association with multiple cofactors. For example, the related proteins, Cdc20 and Cdh1/FZR1, participate in the recognition of APC/C substrates by interacting with specific recognition elements in these substrates (6), called D-boxes (7) and KEN-boxes (8).


Anaphase-promoting complex subunit 11 (APC11) harbors a RING-H2 motif, which is characterized by a series of non-tandem His and Cys residues responsible for the coordination of zinc cations. At the primary amino acid level, APC11 displays sequence similarity to RING-box proteins RBX1 and RBX2, which are the RING-H2 motif-containing subunits of SCF ubiquitin ligase complexes (9). A heterodimer complex containing APC11 and the cullin-like subunit, APC2, forms the catalytic core of the APC/C and is critical for the APC/C to catalyze ubiquitin chain elongation (4,10).


1.  Qiao, X. et al. (2010) Cell Cycle 9, 3904-12.

2.  Harper, J.W. et al. (2002) Genes Dev 16, 2179-206.

3.  Carroll, C.W. and Morgan, D.O. (2002) Nat Cell Biol 4, 880-7.

4.  Gmachl, M. et al. (2000) Proc Natl Acad Sci U S A 97, 8973-8.

5.  Leverson, J.D. et al. (2000) Mol Biol Cell 11, 2315-25.

6.  Kraft, C. et al. (2005) Mol Cell 18, 543-53.

7.  Glotzer, M. et al. (1991) Nature 349, 132-8.

8.  Pfleger, C.M. and Kirschner, M.W. (2000) Genes Dev 14, 655-65.

9.  Chan, A.H. et al. (2001) J Cell Biochem 83, 249-58.

10.  Tang, Z. et al. (2001) Mol Biol Cell 12, 3839-51.


Entrez-Gene Id 51529
Swiss-Prot Acc. Q9NYG5


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
SignalSilence® is a trademark of Cell Signaling Technology, Inc.
XP® is a trademark of Cell Signaling Technology, Inc.