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Western blot analysis of extracts from UV treated HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® ATR siRNA I (+), using Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb #2909 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb confirms reduction of ATR kinase activity, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

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Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® ATR siRNA I (+) or SignalSilence® ATR siRNA II #6289 (+), using ATR Antibody #2790 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The ATR Antibody confirms silencing of ATR expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

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Product Usage Information

CST recommends transfection with 100 nM ATR siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.


Storage: SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.

Product Description

SignalSilence® ATR siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit ATR expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.


Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are PI3 kinase-related kinase (PIKK) family members that phosphorylate multiple substrates on serine or threonine residues that are followed by a glutamine in response to DNA damage or replication blocks (1-3). Despite the essential role of ATR in cell cycle signaling and DNA repair processes, little is known about its activation. Phosphorylation of ATR at serine 428 in response to UV-induced DNA damage has been suggested as a means of activating ATR (4,5). Threonine 1989 was also recently identified as a potential marker of activated ATR (6,7).


1.  Kastan, M.B. and Lim, D.S. (2000) Nat Rev Mol Cell Biol 1, 179-86.

2.  Abraham, R.T. (2004) DNA Repair (Amst) 3, 883-7.

3.  Shechter, D. et al. (2004) DNA Repair (Amst) 3, 901-8.

4.  Vauzour, D. et al. (2007) Arch Biochem Biophys 468, 159-66.

5.  Smith, J. et al. (2010) Adv Cancer Res 108, 73-112.

6.  Nam, E.A. et al. (2011) J Biol Chem 286, 28707-14.

7.  Liu, S. et al. (2011) Mol Cell 43, 192-202.


Entrez-Gene Id 545
Swiss-Prot Acc. Q13535


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
SignalSilence® is a trademark of Cell Signaling Technology, Inc.