Upstream / Downstream

Explore pathways related to this product.

Important Ordering Details

Custom Ordering Details: Product is assembled upon order. Please allow up to three business days for your product to be processed.

Our U.S. Offices Will Be Closing Early

Our U.S. offices will be closing early on November 26th at 6:00 PM EST.

To Purchase # 6332S

6332S 300 µl (3 nmol) $249.00
$ 0. 00

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

REACTIVITY
M

Western blot analysis of extracts from NIH/3T3 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® mTOR siRNA I (Mouse Specific) (+), or SignalSilence® mTOR siRNA II (Mouse Specific) #6342 (+) using mTOR (7C10) Rabbit mAb #2983 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The mTOR (7C10) Rabbit mAb confirms silencing of mTOR expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

Learn more about how we get our images
Image

Product Usage Information

CST recommends transfection with 100 nM SignalSilence® mTOR siRNA I (Mouse Specific) 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.


Storage: SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.

Product Description

SignalSilence® mTOR siRNA I (Mouse Specific) from Cell Signaling Technology (CST) allows the researcher to specifically inhibit mTOR expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.


Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).


1.  Sabers, C.J. et al. (1995) J Biol Chem 270, 815-22.

2.  Brown, E.J. et al. (1994) Nature 369, 756-8.

3.  Sabatini, D.M. et al. (1994) Cell 78, 35-43.

4.  Dennis, P.B. et al. (2001) Science 294, 1102-5.

5.  Gingras, A.C. et al. (2001) Genes Dev. 15, 807-826.

6.  Fang, Y. et al. (2001) Science 294, 1942-5.

7.  Peterson, R.T. et al. (2000) J Biol Chem 275, 7416-23.

8.  Huang, S. and Houghton, P.J. (2003) Curr Opin Pharmacol 3, 371-7.

9.  Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.


Entrez-Gene Id 56717
Swiss-Prot Acc. Q9JLN9


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
SignalSilence® is a trademark of Cell Signaling Technology, Inc.