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Western blot analysis of extracts from C2C12 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® LRP6 siRNA I (Mouse Specific) #9834 (+) or SignalSilence® LRP6 siRNA II (Mouse Specific) (+), using LRP6 (C47E12) Rabbit mAb #3395 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The LRP6 (C47E12) Rabbit mAb confirms silencing of LRP6 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

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Product Usage Information

CST recommends transfection with 100 nM SignalSilence® LRP6 siRNA II (Mouse Specific) 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.


Storage: SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.

Product Description

SignalSilence® LRP6 siRNA II (Mouse Specific) from Cell Signaling Technology (CST) allows the researcher to specifically inhibit LRP6 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.


Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

LRP5 and LRP6 are single-pass transmembrane proteins belonging to the low-density lipoprotein receptor (LDLR)-related protein family. Unlike other members of the LDLR family, LRP5 and LRP6 have four EGF and three LDLR repeats in the extracellular domain, and proline-rich motifs in the cytoplasmic domain (1). They function as co-receptors for Wnt and are required for the canonical Wnt/β-catenin signaling pathway (2,3). LRP5 and LRP6 are highly homologous and have redundant roles during development (4,5). The activity of LRP5 and LRP6 can be inhibited by the binding of some members of the Dickkopf (DKK) family of proteins (6,7). Upon stimulation with Wnt, LRP6 is phosphorylated at multiple sites including Thr1479, Ser1490, and Thr1493 by kinases such as GSK-3 and CK1 (8-10). Phosphorylated LRP6 recruits axin to the membrane and presumably activates β-catenin signaling (8-10).


1.  Brown, S.D. et al. (1998) Biochem. Biophys. Res. Commun. 248, 879-888.

2.  Pinson, K.I. et al. (2000) Nature 407, 535-538.

3.  Tamai, K. et al. (2000) Nature 407, 530-535.

4.  Kelly, O.G. et al. (2004) Development 131, 2803-2815.

5.  He, X. et al. (2004) Development 131, 1663-1677.

6.  Semënov, M.V. et al. (2001) Curr Biol 11, 951-61.

7.  Bafico, A. et al. (2001) Nat. Cell Biol. 3, 683-668.

8.  Tamai, K. et al. (2004) Mol. Cell 13, 149-156.

9.  Zeng, X. et al. (2005) Nature 438, 873-877.

10.  Davidson, G. et al. (2005) Nature 438, 867-872.


Entrez-Gene Id 16974
Swiss-Prot Acc. O88572


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
SignalSilence® is a trademark of Cell Signaling Technology, Inc.