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Product Usage Information

Add 10 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml pre-incubated with biotinylated primary antibody. See protocol for more details.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Product Description

Streptavidin (Sepharose Bead Conjugate) is useful for the precipitation of biotinylated proteins (1,2). Recombinant streptavidin is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads.

Streptavidin is a 53,000 dalton tetrameric protein purified from the bacterium Streptomyces avidinii (3). Each subunit binds to biotin with extremely high affinity. Because of its strong non-covalent interaction with biotin, streptavidin can be used to isolate biotinylated proteins (1,2).

1.  Updyke, T.V. and Nicolson, G.L. (1984) J Immunol Methods 73, 83-95.

2.  Buckie, J.W. and Cook, G.M. (1986) Anal Biochem 156, 463-72.

3.  Chaiet, L. and Wolf, F.J. (1964) Arch Biochem Biophys 106, 1-5.

4.  Reznik, G.O. et al. (1998) Proc Natl Acad Sci USA 95, 13525-13530.

For Research Use Only. Not For Use In Diagnostic Procedures.
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