Upstream / Downstream
Explore pathways related to this product.
CST Antibody Performance Guarantee
Find answers on our FAQs page.
PTM information and tools available.
IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (Sepharose® Bead Conjugate) #4078
This product is discontinued
Gallery: IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (Sepharose® Bead Conjugate) #4078
IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) detects endogenous levels of total IκBα protein.
Monoclonal antibody is produced by immunizing animals with a GST-IκBα fusion protein corresponding the amino-terminus of human IκBα.
This Cell Signalinling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose® beads. IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (Sepharose® Bead Conjugate) is useful for the immunoprecipitation of IκBα protein.
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).
Protein Specific References
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.