News from the Bench
Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.
CST Antibody Performance Guarantee
To Purchase # 9863S
|9863P||200 µl (10 immunoprecipitations)||$64.00|
|9863S||1 ml (50 immunoprecipitations)||$129.00|
|$ 0. 00|
Find answers on our FAQs page.
PTM information and tools available.
Protein A Agarose Beads #9863
Gallery: Protein A Agarose Beads #9863
- Immunoprecipitation (IP) of COS-7 cell extracts using Ubc12 (D13D7) Rabbit mAb #5641 and Protein A Agarose Beads #9863. Western blot analysis was performed on the IP pellet (lane 1) and supernatant (lane 2) using Ubc12 (D13D7) Rabbit mAb #5641.
Vortex tube briefly to resuspend the beads. Add 20-40 μl of bead slurry to each immunoprecipitation (IP) reaction. For bead washing and subsequent elution of immunocomplexes, the beads can be separated from solution by a brief 1 minute centrifugation in a microcentrifuge at 6,000 rpm. Resuspend the beads in solution by gently vortexing or rocking the tube. Please follow CST's recommended IP protocol to perform IP followed by western blot.Storage: Supplied as a 50% slurry in 18.5% ethanol solution. Store at 4°C.
Protein A Agarose Beads are an affinity matrix for the small-scale isolation of immunocomplexes from immunoprecipitations (IP assays). Protein A is covalently coupled to agarose beads. Protein A exhibits high affinity for subclasses of IgG from many species (including human, rabbit, mouse, rat, and sheep) and can be used for immunoprecipitation assays with these antibodies.
20 +/- 2 mg human IgG/ml
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.