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To Purchase # 9863S
|9863P||200 µl (10 immunoprecipitations)|
|9863S||1 ml (50 immunoprecipitations)|
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Protein A Agarose Beads #9863
Gallery: Protein A Agarose Beads #9863
- Immunoprecipitation (IP) of COS-7 cell extracts using Ubc12 (D13D7) Rabbit mAb #5641 and Protein A Agarose Beads #9863. Western blot analysis was performed on the IP pellet (lane 1) and supernatant (lane 2) using Ubc12 (D13D7) Rabbit mAb #5641.
Vortex tube briefly to resuspend the beads. Add 20-40 μl of bead slurry to each immunoprecipitation (IP) reaction. For bead washing and subsequent elution of immunocomplexes, the beads can be separated from solution by a brief 1 minute centrifugation in a microcentrifuge at 6,000 rpm. Resuspend the beads in solution by gently vortexing or rocking the tube. Please follow CST's recommended IP protocol to perform IP followed by western blot.Storage: Supplied as a 50% slurry in 18.5% ethanol solution. Store at 4°C.
Protein A Agarose Beads are an affinity matrix for the small-scale isolation of immunocomplexes from immunoprecipitations (IP assays). Protein A is covalently coupled to agarose beads. Protein A exhibits high affinity for subclasses of IgG from many species (including human, rabbit, mouse, rat, and sheep) and can be used for immunoprecipitation assays with these antibodies.
20 +/- 2 mg human IgG/ml
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.