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Antibody Guarantee

CST Antibody Performance Guarantee


To Purchase # 9863S

9863P 200 µl (10 immunoprecipitations) $64.00
9863S 1 ml (50 immunoprecipitations) $129.00


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Product Usage Information

Vortex tube briefly to resuspend the beads. Add 20-40 μl of bead slurry to each immunoprecipitation (IP) reaction. For bead washing and subsequent elution of immunocomplexes, the beads can be separated from solution by a brief 1 minute centrifugation in a microcentrifuge at 6,000 rpm. Resuspend the beads in solution by gently vortexing or rocking the tube. Please follow CST's recommended IP protocol to perform IP followed by western blot.

Storage: Supplied as a 50% slurry in 18.5% ethanol solution. Store at 4°C. This product is stable for 12 months.

Product Description

Protein A Agarose Beads are an affinity matrix for the small-scale isolation of immunocomplexes from immunoprecipitations (IP assays). Protein A is covalently coupled to agarose beads. Protein A exhibits high affinity for subclasses of IgG from many species (including human, rabbit, mouse, rat, and sheep) and can be used for immunoprecipitation assays with these antibodies.

Binding Capacity:

20 +/- 2 mg human IgG/ml

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Protein A Agarose Beads