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To Purchase # 4326S

4326S 400 µl (40 immunoprecipitations) $329.00


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H M R Mk Dg Endogenous 54 Rabbit IgG

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Specificity / Sensitivity

PTEN (D4.3) XP® Rabbit mAb (Sepharose® Bead Conjugate) detects endogenous levels of total PTEN protein.

Species Reactivity: Human, Mouse, Rat, Monkey, Dog
Species predicted to react based on 100% sequence homology: Chicken

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues in the carboxy-terminal sequence of human PTEN.

Product Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose® beads. PTEN (D4.3) XP® Rabbit mAb (Sepharose® Bead Conjugate) is useful for the immunoprecipitation of PTEN protein.

PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10).

1.  Wan X and Helman LJ (2003) Oncogene 22, 8205–11

2.  Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7.

3.  Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8.

4.  Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91.

5.  Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8.

6.  Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8.

7.  Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30.

8.  Funamoto, S. et al. (2002) Cell 109, 611-23.

9.  Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.

10.  Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.

Entrez-Gene Id 5728
Swiss-Prot Acc. P60484

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

PTEN (D4.3) XP® Rabbit mAb (Sepharose® Bead Conjugate)