News from the Bench

Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.


Antibody Guarantee

CST Antibody Performance Guarantee


To Purchase # 3999S

3999S 1 ml $129.00


Find answers on our FAQs page.


PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


Product Usage Information

Storage: Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Product Description

This Cell Signaling Technology product is useful for the detection of biotinylated proteins (1,2). Conjugation of horseradish peroxidase (HRP) to streptavidin is obtained by cross linking the amino groups on streptavidin with the carbohydrate groups on HRP.

Streptavidin is a 53 kDa homotetramer isolated from Streptomyces avidinii for use in isolation and detection bioassays (3). Each streptavidin subunit forms high affinity non-covalent bonds with the vitamin biotin. Because of its strong non-covalent interaction with biotin, streptavidin can be used to detect and isolate biotinylated proteins (1,2).

Chemiluminescent detection systems have emerged as the best all-around detection method for use with western blots and ELISA. These detection assays eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives. Streptavidin-HRP is used with biotinylated proteins and specific chemiluminescent substrates to generate light signal. Streptavidin-HRP conjugates have a very high turnover rate, coupling high sensitivity with short reaction times.

1.  Updyke, T.V. and Nicolson, G.L. (1984) J Immunol Methods 73, 83-95.

2.  Buckie, J.W. and Cook, G.M. (1986) Anal Biochem 156, 463-72.

3.  Chaiet, L. and Wolf, F.J. (1964) Arch Biochem Biophys 106, 1-5.

4.  Reznik, G.O. et al. (1998) Proc Natl Acad Sci U S A 95, 13525-30.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.