HECT Domain
The HECT domain of E6AP ubiquitin-protein ligase.
Domain Binding and Function
The HECT domain, short for Homologous to the E6-AP Carboxyl Terminus, is an approximately 40 kDa (350 amino acid) catalytic domain found at the carboxy-terminus of Hect-class E3 ubiquitin protein ligases. This domain functions to bind specific E2s, accepts ubiquitin from the E2 to form a ubiquitin-thioester intermediate with the HECT active cysteine, and then transfers ubiquitin to either the ε-amino groups of lysine side chains of the substrate or to the growing end of multiubiquitin chains. The formation of a thioester intermediate with Ub is unique to Hect E3s and has not been observed with other classes of E3s.
Structure Reference
- Huang, L. et al. (1999) Science 286 (5443), 1321–1326.
Examples of Domain Proteins
Binding Examples
| HECT Domain Proteins | Binding Partners |
|---|---|
| E6AP | UbcH7 |
| Nedd4 | UbcH5 |
| HectH7 | UbcH7 |
| HectH6(p532) | UbcH5 |
