Protein Degradation: HECT Domain
The HECT domain of E6AP ubiquitin-protein ligase.
Domain Binding and Function
The HECT domain (Homologous to the E6-AP Carboxyl Terminus) is an approximately 40 kDa (350 amino acid) catalytic domain found at the carboxy-terminus of HECT-class E3 ubiquitin protein ligases. This domain binds specific E2 enzymes, accepts ubiquitin from E2 to form an ubiquitin-thioester intermediate with the HECT active cysteine, and then transfers ubiquitin to either the ε-amino groups of lysine side chains of the substrate or to the growing end of multiubiquitin chains. The formation of a thioester intermediate with ubiquitin is unique to HECT E3 ligases and is not seen with other types of E3 ligase.
- Huang, L. et al. (1999) Science 286 (5443), 1321–1326.
Examples of Domain Proteins
|HECT Domain Proteins||Binding Partners|