Protein Acetylation

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• Reviews
• Pathway Key
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Pathway Description:

Protein acetylation plays a crucial role in regulating chromatin structure and transcriptional activity. Many transcriptional coactivators possess intrinsic acetylase activity, while transcriptional corepressors are associated with deacetylase activity. Acetylation complexes (such as CBP/p300 and PCAF) or deacetylation complexes (such as Sin3, NuRD, NcoR and SMRT) are recruited to DNA-bound transcription factors (TFs) in response to signaling pathways. Histone hyperacetylation by histone acetyltransferases (HATs) is associated with transcriptional activation, whereas histone deacetylation by histone deacetylases (HDACs) is associated with transcriptional repression. Histone acetylation stimulates transcription by remodeling higher order chromatin structure, weakening histone-DNA interactions, and providing binding sites for transcriptional activation complexes containing proteins that possess bromodomains, which bind acetylated lysine. Histone deacetylation represses transcription through an inverse mechanism involving the assembly of compact higher order chromatin and the exclusion of bromodomain-containing transcription activation complexes. Histone hypoacetylation is a hallmark of silent heterochromatin. Site-specific acetylation of a growing number of non-histone proteins, including p53 and E2F, has been shown to regulate their activity, localization, specific interactions as well as stability/degradation, therefore controlling a variety of cellular processes, such as transcription, proliferation, apoptosis and differentiation. At an organismal level, acetylation plays an important role in immunity, circadian rhythmicity and memory formation. Protein acetylation is becoming a favorable target in drug design for numerous disease conditions.

Selected Reviews:

• Dali-Youcef N, Lagouge M, Froelich S, Koehl C, Schoonjans K, Auwerx J (2007) Sirtuins: the 'magnificent seven', function, metabolism and longevity. Ann. Med. 39(5), 335–45.
• Hadnagy A, Beaulieu R, Balicki D (2008) Histone tail modifications and noncanonical functions of histones: perspectives in cancer epigenetics. Mol. Cancer Ther. 7(4), 740–8.
• Olsson A, Manzl C, Strasser A, Villunger A (2007) How important are post-translational modifications in p53 for selectivity in target-gene transcription and tumour suppression? Cell Death Differ. 14(9), 1561–75.
• Tyteca S, Legube G, Trouche D (2006) To die or not to die: a HAT trick. Mol. Cell 24(6), 807–8.
• Yang XJ, Seto E (2007) HATs and HDACs: from structure, function and regulation to novel strategies for therapy and prevention. Oncogene 26(37), 5310–8.