Product Pathways - Motif Antibodies
PTMScan® Phospho-CDK Substrate Motif (K/RS*PXK/R) Immunoaffinity Beads
| Consenus Site | Cell or Tissue Type | Study No. | Modified Peptides Identified |
|---|---|---|---|
| (K/R)S*PX(K/R) | HeLa (human epithelial carcinoma) | 2724 | 305 PDF XLS |
Services Information
This product is not for individual sale. It is only available as a component of the PTMScan® Proteomics System. PTMScan® Proteomics System orders must be priced out individually. Please email us at ptmscan@cellsignal.com to receive the most accurate pricing.
Description
PTMScan® Immunoaffinity Beads are custom preparations of motif antibodies coupled to protein A beads. They are intended only for use for PTMScan® and are available as components of the PTMScan® Proteomics System.
Specificity / Sensitivity
PTMScan® Phospho-CDK Substrate Motif (K/RS*PXK/R) Immunoaffinity Beads detect and capture endogenous levels of peptide derived from protease digested cellular proteins containing phospho-Ser in a (K/R)(S*)PX(K/R) motif. Some cross-reactivity is observed for peptides containing phospho-Thr followed by Pro only. The antibody does not cross-react with phospho-Thr- or phospho-Tyr-containing peptides/proteins. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or nocodazole-treated, using Phospho-(Ser) CDKs Substrate Antibody, the unconjugated antibody corresponding to PTMScan® Phospho-CDK Substrate Motif (K/RS*PXK/R) Immunoaffinity Beads.
Background
Cyclin-dependent kinases (CDKs) are a family of Ser/Thr kinases that regulate cell-cycle transitions through their association and subsequent phosphorylation of targets in a strictly ordered fashion (1). The substrates for CDKs are proline-directed. The consensus amino acid sequence for CDK substrate is (K/R)(S*)PX(K/R), where X denotes any one of the 20 amino acids (2-4) and S* is the phosphorylation site. Phospho-(Ser) CDKs Substrate Antibody recognizes phosphorylated CDK substrates at their consensus motif, providing a powerful tool for CDK target discovery and characterization, as well as HTS drug screening for potential kinase regulators.
- Morgan, D.O. (1997) Annu Rev Cell Dev Biol 13, 261-91.
- Songyang, Z. et al. (1996) Mol Cell Biol 16, 6486-93.
- Songyang, Z. (1999) Prog Biophys Mol Biol 71, 359-72.
- Holmes, J.K. and Solomon, M.J. (1996) J Biol Chem 271, 25240-6.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
