Product Pathways - Motif Antibodies
PTMScan® Phospho-MAPK/CDK Substrate Motif (PXS*P, S*PXK/R) Immunoaffinity Beads
| Consenus Site | Cell or Tissue Type | Study No. | Modified Peptides Identified |
|---|---|---|---|
| PXS*P, S*PX(K/R) | HeLa (human epithelial carcinoma) | 1915 | 459 PDF XLS |
Services Information
This product is not for individual sale. It is only available as a component of the PTMScan® Proteomics System. PTMScan® Proteomics System orders must be priced out individually. Please email us at ptmscan@cellsignal.com to receive the most accurate pricing.
Description
PTMScan® Immunoaffinity Beads are custom preparations of motif antibodies coupled to protein A beads. They are intended only for use for PTMScan® and are available as components of the PTMScan® Proteomics System.
Specificity / Sensitivity
PTMScan® Phospho-MAPK/CDK Substrate Motif (PXS*P, S*PXK/R) Immunoaffinity Beads detect and capture endogenous levels of peptide derived from protease digested cellular proteins containing phospho-Ser in a PXS*P or S*PXK/R motif, as well as a PXpSPXR/K motif. The antibody is phospho-specific, and does not react with phospho-Thr- or phospho-Tyr-containing peptides/proteins. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or nocodazole-treated, using Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb, the unconjugated antibody corresponding to PTMScan® Phospho-MAPK/CDK Substrate Motif (PXS*P, S*PXK/R) Immunoaffinity Beads.
Background
The MAPK and CDK families of serine/threonine protein kinases play important roles in cell signaling and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-6). To study and discover new MAPK and CDK substrates, Cell Signaling Technology has developed antibodies that bind to phospho-threonine followed by proline.
- Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
- Seger, R. and Krebs, E.G. (1995) FASEB J 9, 726-35.
- Nurse, P. (2000) Cell 100, 71-8.
- Cross, T.G. et al. (2000) Exp Cell Res 256, 34-41.
- Yang, C.C. et al. (1998) J Protein Chem 17, 329-35.
- Reynolds, C.H. et al. (2000) J Neurochem 74, 1587-95.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
