Product Pathways - Motif Antibodies
PTMScan® Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads
|Consenus Site||Cell or Tissue Type||Study No.||Modified Peptides Identified|
|(K/R)(K/R)X(S*/T*)||PC-12 (rat adrenal pheochromocytoma cell line)||2929||205 PDF XLS|
This product is not for individual sale. It is only available as a component of the PTMScan® Proteomics System. PTMScan® Proteomics System orders must be priced out individually. Please email us at firstname.lastname@example.org to receive the most accurate pricing.
PTMScan® Immunoaffinity Beads are custom preparations of motif antibodies coupled to protein A beads. They are intended only for use in PTMScan® and are available as components of the PTMScan® Proteomics System.
Specificity / Sensitivity
PTMScan® Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads detect and capture endogenous levels of peptide derived from protease digested cellular proteins containing a phospho-Ser/Thr residue with Arg at the -3 and -2 positions. To a lesser extent, the antibody recognizes other -3 Arg-bearing phospho-Ser/Thr peptides, such as substrates for Akt and PKC. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Phospho-PKA substrate specific antibodies from Cell signaling Technology are powerful tools for investigaing the regulation of phosphorylation by PKA and other Arg-directed kinases.
- Montminy, M. (1997) Annu Rev Biochem 66, 807-22.
- Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.