Product Pathways - Motif Antibodies
PTMScan® Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads
|Consenus Site||Cell or Tissue Type||Study No.||Modified Peptides Identified|
|(K/R)(K/R)X(S*/T*)||PC-12 (rat adrenal pheochromocytoma cell line)||2929||205 PDF XLS|
This product is not for individual sale. It is only available as a component of the PTMScan® Proteomics System. PTMScan® Proteomics System orders must be priced out individually. Please email us at email@example.com to receive the most accurate pricing.
PTMScan® Immunoaffinity Beads are custom preparations of motif antibodies coupled to protein A beads. They are intended only for use in PTMScan® and are available as components of the PTMScan® Proteomics System.
Specificity / Sensitivity
PTMScan® Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads detect and capture endogenous levels of peptide derived from protease digested cellular proteins containing a phospho-Ser/Thr residue with Arg at the -3 and -2 positions. To a lesser extent, the antibody recognizes other -3 Arg-bearing phospho-Ser/Thr peptides, such as substrates for Akt and PKC. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Phospho-PKA substrate specific antibodies from Cell signaling Technology are powerful tools for investigaing the regulation of phosphorylation by PKA and other Arg-directed kinases.
- Montminy, M. (1997) Annu Rev Biochem 66, 807-22.
- Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
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For Research Use Only. Not For Use In Diagnostic Procedures.