Product Pathways - Motif Antibodies
PTMScan® Phospho-Thr-X-Arg Motif (T*XR) Immunoaffinity Beads
| Consenus Site | Cell or Tissue Type | Study No. | Modified Peptides Identified |
|---|---|---|---|
| T*X(K/R) | HeLa (human epithelial carcinoma) | 2655 | 82 PDF XLS |
Services Information
This product is not for individual sale. It is only available as a component of the PTMScan® Proteomics System. PTMScan® Proteomics System orders must be priced out individually. Please email us at ptmscan@cellsignal.com to receive the most accurate pricing.
Description
PTMScan® Immunoaffinity Beads are custom preparations of motif antibodies coupled to protein A beads. They are intended only for use for PTMScan® and are available as components of the PTMScan® Proteomics System.
Specificity / Sensitivity
PTMScan® Phospho-Thr-X-Arg Motif (T*XR) Immunoaffinity Beads detect and capture endogenous levels of peptide derived from protease digested cellular proteins containing the phospho-Thr-X-Arg motif. Pro is preferred but not necessary at +1 position. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or nocodazole-treated, using Phospho-Threonine-X-Arginine Antibody, the unconjugated antibody corresponding to PTMScan® Phospho-Thr-X-Arg Motif (T*XR) Immunoaffinity Beads.
Background
Some signaling molecules can be regulated by phosphorylation at a specific threonine followed by arginine or lysine at the +2 position. For example, conventional PKC isozymes phosphorylate substrates containing serine or threonine with Arg or Lys at the -3, -2 and +2 positions (1-2). c-Raf, a mitogen-activated protein kinase and the main effector recruited by GTP-bound Ras, is phosphorylated at Thr481 and Thr491 followed by Lys at the +2 position (3). Phosphorylation of these sites is important for enzyme activities. To determine the phosphorylation state of Thr in the Thr-X-Arg motif, and to identify potential new phosphorylation sites with this motif, Cell Signaling Technology has developed a Phospho-Threonine X-Arginine Antibody that recognizes phosphorylated Thr followed by Arg or Lys at the +2 position.
- Nishikawa, K. et al. (1997) J Biol Chem 272, 952-60.
- Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
- Zhang, B.H. and Guan, K.L. (2000) EMBO J 19, 5429-39.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
