Product Pathways - Motif Antibodies
PTMScan® Phospho-Thr-X-Arg Motif (T*XR) Immunoaffinity Beads
|Consenus Site||Cell or Tissue Type||Study No.||Modified Peptides Identified|
|T*X(K/R)||HeLa (human epithelial carcinoma)||2655||82 PDF XLS|
This product is not for individual sale. It is only available as a component of the PTMScan® Proteomics System. PTMScan® Proteomics System orders must be priced out individually. Please email us at email@example.com to receive the most accurate pricing.
PTMScan® Immunoaffinity Beads are custom preparations of motif antibodies coupled to protein A beads. They are intended only for use for PTMScan® and are available as components of the PTMScan® Proteomics System.
Specificity / Sensitivity
PTMScan® Phospho-Thr-X-Arg Motif (T*XR) Immunoaffinity Beads detect and capture endogenous levels of peptide derived from protease digested cellular proteins containing the phospho-Thr-X-Arg motif. Pro is preferred but not necessary at +1 position. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Some signaling molecules can be regulated by phosphorylation at a specific threonine followed by arginine or lysine at the +2 position. For example, conventional PKC isozymes phosphorylate substrates containing serine or threonine with Arg or Lys at the -3, -2 and +2 positions (1-2). c-Raf, a mitogen-activated protein kinase and the main effector recruited by GTP-bound Ras, is phosphorylated at Thr481 and Thr491 followed by Lys at the +2 position (3). Phosphorylation of these sites is important for enzyme activities. To determine the phosphorylation state of Thr in the Thr-X-Arg motif, and to identify potential new phosphorylation sites with this motif, Cell Signaling Technology has developed a Phospho-Threonine X-Arginine Antibody that recognizes phosphorylated Thr followed by Arg or Lys at the +2 position.
- Nishikawa, K. et al. (1997) J Biol Chem 272, 952-60.
- Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
- Zhang, B.H. and Guan, K.L. (2000) EMBO J 19, 5429-39.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.