Product Pathways - Motif Antibodies

• Antibodies for PTMScan^®
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• application references

PTMScan^® Ubiquitin Branch Motif (K-ε-GG) Immunoaffinity Beads

Services Information

This product is not for individual sale. It is only available as a component of the PTMScan^® Proteomics System. PTMScan^® Proteomics System orders must be priced out individually. Please email us at ptmscan@cellsignal.com to receive the most accurate pricing.

Description

PTMScan^® Immunoaffinity Beads are custom preparations of motif antibodies coupled to protein A beads. They are intended only for use for PTMScan^® and are available as components of the PTMScan^® Proteomics System.

Specificity / Sensitivity

PTMScan^® Ubiquitin Branch Motif (K-ε-GG) Immunoaffinity Beads detect and capture endogenous levels of peptide derived from trypsin digested cellular proteins containing ubiquitinated (mono or poly ubiquitinated) or neddylated proteins. The antibody recognizes peptides containing (K-ε-GG) motif in a wide range of sequence contexts, no preference is observed at any positions. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Background

Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH₂ of the target protein lysine residue (1-3). The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities. Several proteins such as IκB, p53, cdc25A, and Bcl-2 have been shown to be targets for the ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis (4-7).

1. Ciechanover, A. (1998) EMBO J. 17, 7151-7160.
2. Hochstrasser, M. (2000) Nat. Cell Biol. 2, E153-E157.
3. Hochstrasser, M. (2000) Science 289, 563-564.
4. Bernardi, R. et al. (2000) Oncogene 19, 2447-2454.
5. Aberle, H. et al. (1997) EMBO J. 16, 3797-3804.
6. Salomoni, P. and Pandolfi, P.P. (2002) Nat. Cell Biol. 4, E152-E153.
7. Jesenberger, V. and Jentsch, S. (2002) Nat. Rev. Mol. Cell Biol. 3, 112-121.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

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For Research Use Only. Not For Use In Diagnostic Procedures.