Product Pathways - Motif Antibodies
PTMScan® Phospho-Tyr Motif (Y*) Immunoaffinity Beads
| Consenus Site | Cell or Tissue Type | Study No. | Modified Peptides Identified |
|---|---|---|---|
| Y* | H3255 (non-small cell lung cancer) | 8809 | 930 PDF XLS |
Services Information
This product is not for individual sale. It is only available as a component of the PTMScan® Proteomics System. PTMScan® Proteomics System orders must be priced out individually. Please email us at ptmscan@cellsignal.com to receive the most accurate pricing.
Description
PTMScan® Immunoaffinity Beads are custom preparations of motif antibodies coupled to protein A beads. They are intended only for use for PTMScan® and are available as components of the PTMScan® Proteomics System.
Specificity / Sensitivity
PTMScan® Phospho-Tyr Motif (Y*) Immunoaffinity Beads bind detect and capture endogenous levels of peptide derived from protease digested cellular proteins containing phospho-Tyr residues in a manner largely independent of the surrounding amino acid sequence. The antibody does not cross-react with peptides containing phospho-Ser or phospho-Thr residues. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or EGF-treated, using Phospho-Tyrosine Mouse mAb (P-Tyr-100), the unconjugated antibody corresponding to PTMScan® Phospho-Tyr Motif (Y*) Immunoaffinity Beads.
Western Blotting
Western blot analysis of extracts from H3255 cells, untreated or treated with gefinitib, using Phospho-Tyrosine Mouse mAb (P-Tyr-100), the unconjugated antibody corresponding to PTMScan® Phospho-Tyr Motif (Y*) Immunoaffinity Beads.
Background
Tyrosine phosphorylation plays a key role in cellular signaling (1). Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.
- Schlessinger, J. (2000) Cell 103, 211-25.
- Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
- Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9.
- Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
