Product Pathways - Motif Antibodies

• Antibodies for PTMScan^®
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• application references

PTMScan^® Phospho-CK Substrate Motif (S*/T*D/EXD/E) Immunoaffinity Beads

Services Information

This product is not for individual sale. It is only available as a component of the PTMScan^® Proteomics System. PTMScan^® Proteomics System orders must be priced out individually. Please email us at ptmscan@cellsignal.com to receive the most accurate pricing.

Description

PTMScan^® Immunoaffinity Beads are custom preparations of motif antibodies coupled to protein A beads. They are intended only for use for PTMScan^® and are available as components of the PTMScan^® Proteomics System.

Specificity / Sensitivity

PTMScan^® Phospho-CK Substrate Motif (S*/T*D/EXD/E) Immunoaffinity Beads detect and capture endogenous levels of peptide derived from protease digested cellular proteins containing a S*/T*D/EXD/E motif which is the CKII phosphorylation consensus sequence. The antibody is useful tool to study CKII substrates. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Background

Protein kinase CK2 (casein kinase II) is a highly conserved protein-serine/threonine kinase that is ubiquitously expressed in eukaryotic organisms. It is a tetrameric serine-threonine kinase comprised of two α or α' catalytic subunits of 42-44 and 38 kDa, and two β regulatory subunits of 28 kDa (1-3). CK2 functions in a variety of cellular processes, including cell cycle progression, apoptosis, transcription, and viral infection; the kinase plays a role in human cancers and other diseases (4-7). Over 100 distinct CK2 substrates have been identified. CK2 phosphorylation is specified by multiple acidic residues (Glu or Asp) located downstream (+1 to +5) from the phosphorylated serine or threonine residues. The residue at position +3 is most crucial, while the residue at +1 is the second most important position (8).

1. Meisner, H. et al. (1989) Biochemistry 28, 4072-6.
2. Seldin, D.C. and Leder, P. (1995) Science 267, 894-7.
3. Litchfield, D.W. (2003) Biochem J 369, 1-15.
4. Pinna, L.A. and Allende, J.E. (2009) Cell Mol Life Sci 66, 1795-9.
5. St-Denis, N.A. and Litchfield, D.W. (2009) Cell Mol Life Sci 66, 1817-29.
6. Dominguez, I. et al. (2009) Cell Mol Life Sci 66, 1850-7.
7. Trembley, J.H. et al. (2009) Cell Mol Life Sci 66, 1858-67.
8. Meggio, F. and Pinna, L.A. (2003) FASEB J 17, 349-68.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

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For Research Use Only. Not For Use In Diagnostic Procedures.