Product Pathways - Motif Antibodies
PTMScan® Phospho-Thr-Pro Motif (T*P) Immunoaffinity Beads
|Consenus Site||Cell or Tissue Type||Study No.||Modified Peptides Identified|
|T*P, T*PP||HeLa (human epithelial carcinoma)||10686, 10689||578 PDF XLS|
This product is not for individual sale. It is only available as a component of the PTMScan® Proteomics System. PTMScan® Proteomics System orders must be priced out individually. Please email us at email@example.com to receive the most accurate pricing.
PTMScan® Immunoaffinity Beads are custom preparations of motif antibodies coupled to protein A beads. They are intended only for use for PTMScan® and are available as components of the PTMScan® Proteomics System.
Specificity / Sensitivity
PTMScan® Phospho-Thr-Pro Motif (T*P) Immunoaffinity Beads detect and capture endogenous levels of peptide derived from protease digested cellular proteins containing phospho-Thr only when followed by Pro. Some preference of Pro at +2 position is observed (e.g., phospho-Elk-1). (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Proline-directed protein kinases phosphorylate Ser and Thr residues prior to Pro residues. MAP kinases and cyclin dependent kinases (CDKs) belong to this family of kinases. Proline-directed phosphorylation is one of the major regulatory phosphorylation events in many cellular processes such as cell proliferation and differentiation (1-6). More than a quarter of the sites identified in global phosphorylation studies are phosphorylated at pSer-Pro or pThr-Pro sites (7), suggesting proline-directed protein kinases regulate a large number of proteins. Cell Signaling Technology’s pThr-Pro Antibody provides a useful tool to study the substrates of proline-directed kinases.
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For Research Use Only. Not For Use In Diagnostic Procedures.