Product Pathways - Motif Antibodies
PTMScan® Phospho-PKC Substrate Motif (K/RXS*XK/R) Immunoaffinity Beads
| Consenus Site | Cell or Tissue Type | Study No. | Modified Peptides Identified |
|---|---|---|---|
| (K/R)XS*X(K/R) |
Services Information
This product is not for individual sale. It is only available as a component of the PTMScan® Proteomics System. PTMScan® Proteomics System orders must be priced out individually. Please email us at ptmscan@cellsignal.com to receive the most accurate pricing.
Description
PTMScan® Immunoaffinity Beads are custom preparations of motif antibodies coupled to protein A beads. They are intended only for use for PTMScan® and are available as components of the PTMScan® Proteomics System.
Specificity / Sensitivity
PTMScan® Phospho-PKC Substrate Motif (K/RXpSXK/R) Immunoaffinity Beads detects endogenous levels of peptides and proteins containing phospho-Ser residues surrounded by Arg or Lys at the -2 and +2 positions. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Western Blotting
Western blot analysis of Jurkat cells, untreated or treated with TPA #4174, using Phospho-PKC Substrate (K/RXS*XK/R) Rabbit mAb #6967, the unconjugated antibody corresponding to PTMScan® Phospho-PKC Substrate Motif (K/RXS*XK/R) Immunoaffinity Beads.
Background
Although protein kinase C (PKC) family members are involved in a number of signal transduction processes including secretion, gene expression, proliferation, and muscle contraction (1,2), many of their substrates remain unidentified. Isozymes of PKC are subdivided into conventional PKCs (cPKC), novel PKCs (nPKC), and atypical PKCs (aPKC). PKCα, βI, βII, and γ isoforms belong to the cPKC group (1). When activated, cPKC isozymes phosphorylate substrates containing Ser or Thr, with Arg or Lys at the -3, -2, and +2 positions, and a hydrophobic amino acid at position +1 (1-3). To identify potential PKC substrates, Cell Signaling Technology has developed antibodies that recognize this motif. Phospho-(Ser) PKC Substrate (D13C5/D47H10/D50E3) Rabbit mAb is specific to cPKC substrates containing phospho-Ser.
- Nishikawa, K. et al. (1997) J Biol Chem 272, 952-60.
- Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
- Obata, T. et al. (2000) J Biol Chem 275, 36108-15.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
