PTMScan® Direct Multi-Pathway Service
PTMScan® Direct Multi-Pathway Service allows access to perform targeted screening and quantification of a defined set of protein sites and signaling nodes critical for drug discovery, biomarker discovery, or diagnostic development. The PTMScan Direct Multi-Pathway Service spans a large number of pathways, including Akt, MAPK, NF-κB, and Jak/Stat signaling pathways. The table below illustrates the list of protein sites that can be monitored with PTMScan® Direct Multi-Pathway Service.
Altogether, the PTMScan® Direct Multi-Pathway service provides a robust method to qualitatively identify and quantitatively profile 111 unique phosphorylation sites to 56 proteins.
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Protein Sites are sorted alphabetically by primary protein.
| PTMScan® Direct Multi-Pathway Protein Sites | |||
|---|---|---|---|
| PTMScan® Direct Multi-Pathway Protein Sites | |||
| 4EBP-2 (Thr37) | 4EBP-2 (Thr46) | β-Actin* | Akt1 (Ser473) |
| Akt1 (Thr308) | Akt1 (Thr312) | Akt1 (Thr479) | Akt2 (Ser474) |
| Akt2 (Ser478) | Akt2 (Thr309) | Anillin (Thr364) | ATF-2 (Thr62) |
| ATF-2 (Thr69) | ATF-2 (Thr71) | Aurora A (Thr288) | Aurora B (Thr232) |
| Aurora C (Thr198) | Bad (Ser75) | Caveolin-2 (Ser18) | Caveolin-2 (Ser20) |
| cdc2 (Tyr15) | cdc25C (Ser168) | cdc25C (Ser216) | CDK1 (Tyr15) |
| CDK5 (Tyr15) | Chk1 (Ser345) | Claudin-4 (Tyr208) | CREB (Ser133) |
| DOT1L (Ser741) | DOT1L (Thr751) | FGFR4 (Tyr642) | FGFR4 (Tyr643) |
| GADPH* | GSK-3α (Ser21) | GSK-3β (S9) | Histone H3* |
| hnRNP LL (Ser61) | IκBα (Ser32) | IκBα (Ser36) | IκBα (Tyr42) |
| c-Jun (Ser73) | JunD (Ser100) | JunD (Ser100) | JunD (Ser90) |
| LMO7 (Ser318) | LMO7 (Ser322) | LMO7 (Tyr323) | LRP6 (Ser1490) |
| MDM2 (Ser166) | MEK1 (Ser217) | MEK1 (Ser221) | MEK2 (Ser222) |
| MEK2 (Ser226) | MKP-2 (Ser386) | mTOR (Ser2448) | OSR1 (Thr185) |
| p42 MAPK (Thr184) | p42 MAPK (Tyr186) | p44 MAPK (Thr202) | p44 MAPK (Tyr204) |
| p70 S6 Kinase (Thr252) | p70 S6 Kinase (Thr412) | p70 S6 Kinase β (Thr228) | p85A (Tyr467) |
| p90RSK (Ser363) | p90RSK (Thr359) | PCH3 (Ser616) | PCH3 (Thr609) |
| PDGFRα (Tyr849) | PDK1 (Ser241) | PI3 Kinase p55 (Tyr199) | PI3 Kinase p85α (Tyr467) |
| PI3 Kinase p85β (Tyr464) | PKCA (Thr496) | PKN1 (Ser916) | PRAS40 (Thr246) |
| Pyk2 (Tyr579) | Pyk2 (Tyr580) | B-Raf (Ser446) | c-Raf (Ser338) |
| Rb (Ser780) | Rb (Ser788) | Rb (Ser795) | Rictor (Ser1370) |
| Rictor (Ser1373) | Rictor (Thr1376) | RIP2 (Ser176) | RSK2 (Ser227) |
| RSK4 (Ser389) | S6 (Ser235) | S6 (Ser236) | S6 (Ser240) |
| S6 (Ser244) | SAPK/JNK1 (Thr183) | SAPK/JNK1 (Tyr185) | JNK3 (Thr221) |
| JNK3 (Thr223) | Smad1 (Ser463) | Smad1 (Ser465) | Stat3 (Ser701) |
| Stat3 (Ser727) | Stat3 (Tyr705) | Stat5 (Tyr694) | Stat6 (Tyr641) |
| TBC1D1 (Ser237) | Timeless (Ser1149) | α-Tubulin* | Wee1 (Ser642) |
| ZNF598 (Ser288) | ZNF828 (Ser405) | Zyxin (Tyr172) | |
* The following targets are included as control markers for total protein: β-Actin, Histone H3, GAPDH, and α-Tubulin.
PTMScan® Direct Multi-Pathway Service Sample Data
Sample Case Study Data
Note: The case studies featured below have been saved in Excel (XLS) format.
