KinomeView™ Profiling

KinomeView™ Services – Ser/Thr Phosphoproteomics

The serine/threonine phosphoproteome is extremely complex. KinomeView™ is a western blotting service in which we use our Phospho-Motif Antibodies to provide a kinome-wide view of cellular phosphorylation. Cell Signaling Technology (CST) has developed 17 Phospho-Motif Antibodies that have broad immunoreactivity for serine, threonine and tyrosine phosphorylation mediated by both known and unknown kinases. These antibodies are used sequentially in KinomeView western blotting and then PhosphoScan^® mass spectrometry studies.

The objective of KinomeView is to determine which Phospho-Motif Antibodies detect phosphorylation that is responsive to the treatment under investigation. In KinomeView Services, we analyze sets of up to 14 client samples with 17 Phospho-Motif Antibodies plus controls. High-resolution western images are generated on the LI-COR Odyssey^®, and the results determine which Phospho-Motif Antibodies to use in PhosphoScan mass spectrometry analysis. The Phospho-Motif Antibodies that reveal changes in banding pattern with treatment in KinomeView will be the same antibodies used in PhosphoScan for LC-MS/MS analysis to identify and quantify hundreds to over a thousand phosphorylation sites and parent phosphoproteins.

Illustration of Phospho-Motif Antibody Coverage of the Kinome

Instructions: Click on a western blot image in the kinome below for a larger image and brief explanation of the proof-of-concept study shown. To zoom or print: right-click in the graphic for Windows, or control-click in the graphic for Macintosh.