KinomeView® Services – Ser/Thr Phosphoproteomics
The serine/threonine phosphoproteome is extremely complex. KinomeView® is a western blotting service in which we use our Phospho-Motif Antibodies to provide a kinome-wide view of cellular phosphorylation. Cell Signaling Technology has developed Phospho-Motif Antibodies that have broad immunoreactivity for serine, threonine, and tyrosine phosphorylation mediated by both known and unknown kinases. These antibodies can be employed sequentially in KinomeView western blotting followed by PhosphoScan® mass spectrometry studies.
The objective of KinomeView is to determine which Phospho-Motif Antibodies detect phosphorylation that is responsive to the treatment under investigation. In KinomeView Services, we analyze sets of up to 14 client samples with Phospho-Motif Antibodies plus controls. High-resolution western images are generated on the LI-COR Odyssey®, and the results determine which Phospho-Motif Antibodies employ subsequent PhosphoScan mass spectrometry analysis. The Phospho-Motif Antibodies that reveal changes in banding pattern with treatment in KinomeView will be the same antibodies used in PhosphoScan for LC-MS/MS analysis to identify and quantify hundreds to over a thousand phosphorylation sites and parent phosphoproteins.
Illustration of Phospho-Motif Antibody Coverage of the Kinome