MethylScan® (Methylation Proteomics)
Histone methylation is crucial for proper programming of the genome during development, and misregulation of the methylation machinery can lead to diseased state including cancer. MethylScan® Service is a PTMScan® Service offering that provides a unique and unparalleled strategy for global analysis of methyltransferase and demethylase activity on protein methylation. The Service utilizes antibodies with high affinity to mono-methylated arginine and mono-methylated lysine peptides for enrichment of mono-methylated peptides from protease-digested cell or tissue samples, followed by LC-MS/MS analysis for quantitative profiles of many hundreds of non-redundant mono-methylated sequences. Reagents for detection of di-methylated and tri-methylated peptides are currently in development. Please contact email@example.com for more information.
MethylScan® Workflow Diagram 0.6 MB
Example Studies and Deliverables
|Description||Antibody||Kit||Consenus Site||LCMS Data & Output Results|
|Mono-Methylated-Arginine||8015||Me-R||Mouse Embryo XLS RAW|
|Mono-Methylated-Arginine||8711||Me-RGG||Mouse Embryo XLS RAW|