MethylScan^® (Methylation Proteomics)

• Ser/Thr Phosphorylation
• Tyr Phosphorylation
• Protein Acetylation
• Ubiquitination
• Methylation

Histone methylation is crucial for proper programming of the genome during development, and misregulation of the methylation machinery can lead to diseased state including cancer. MethylScan^® Service is a new PTMScan^® Service offering that provides a unique and unparalleled strategy for global analysis of methyltransferase and demethylase activity on protein methylation. The Service utilizes antibodies with high affinity to mono-methylated arginine peptides for enrichment of mono-methylated peptides from protease-digested cell or tissue samples, followed by LC-MS/MS analysis for quantitative profiles of many hundreds of non-redundant mono-methylated sequences. Reagents for detection of di-methylated and tri-methylated peptides are currently in development. Please contact ptmscan@cellsignal.com for more information.

MethylScan^® Workflow Diagram  0.6 MB

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Example Studies and Deliverables