AcetylScan® (Acetylation Proteomics)
Histone acetyltransferases (HATs) and histone deacetylases (HDACs) have regulator functions in numerous aspects of cell physiology. AcetylScan® is a unique and unparalleled strategy for global analysis of HDAC and HAT activity on protein acetylation. Our AcetylScan Services utilize antibodies with high affinity to acetylated-lysine (Ac-K) peptides for enrichment of acetylated peptides from protease digested cell samples, followed by LC-MS/MS analysis for quantitative profiles of many hundreds of non-redundant acetylated sequences.
AcetylScan® Services have been applied to class I, II and III HDAC studies, and have been performed on sub-cellular compartment fractions for substrate, target pathway and compound biomarker discovery.
AcetylScan® Workflow Diagram 0.5 MB
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Example Studies and Deliverables
| Target | Antibody | Bead | Kit | Consenus Site | Cell or Tissue Type | Peptides Identified |
|---|---|---|---|---|---|---|
| Acetylated-Lysine | 9814 | 1989 | Ac-K | Jurkat (T cell acute lymphoblastic leukemia) | 283 |
