UbiScan® (Ubiquitination Proteomics)
Ubiquitination is gaining growing attention as an important mechanism for both signal transduction and proteolytic degradation. The UbiScan® ubiquitination proteomics method is a unique and unparalleled strategy for global analysis of cellular ubiquitination. CST has developed a proprietary ubiquitin branch (“K-GG”) XP® monoclonal antibody with specificity for a di-glycine tag that is the remnant of ubiquitin left on protein substrates after trypsin digestion. The “K-GG” XP® monoclonal antibody was generated using CST’s proprietary XMT® monoclonal technology.
In UbiScan Services, we employ the K-GG antibody on trypsin digested cell samples to enrich ubiquitinated peptides, followed by LC-MS/MS analysis for quantitative profiles of hundreds to over a thousand non-redundant ubiquitinated sequences. UbiScan® Proteomic Services are applied in comparative studies to identify substrates and biomarkers of ubiquitin pathway targets and compounds.
UbiScan® Workflow Diagram 0.4 MB
Example Studies and Deliverables
|Description||Antibody||Kit||Consenus Site||LCMS Data & Output Results|
|Ubiquitin Branch||3925||5562||K-ε-GG||Mouse Liver XLS RAW|