UbiScan^® (Ubiquitination Proteomics)

• Ser/Thr Phosphorylation
• Tyr Phosphorylation
• Protein Acetylation
• Ubiquitination
• Methylation

Ubiquitination is gaining growing attention as an important mechanism for both signal transduction and proteolytic degradation. The UbiScan^® ubiquitination proteomics method is a unique and unparalleled strategy for global analysis of cellular ubiquitination. CST has developed a proprietary ubiquitin branch (“K-GG”) XP^® monoclonal antibody with specificity for a di-glycine tag that is the remnant of ubiquitin left on protein substrates after trypsin digestion. The “K-GG” XP^® monoclonal antibody was generated using CST’s proprietary XMT^® monoclonal technology.

In UbiScan Services, we employ the K-GG antibody on trypsin digested cell samples to enrich ubiquitinated peptides, followed by LC-MS/MS analysis for quantitative profiles of hundreds to over a thousand non-redundant ubiquitinated sequences. UbiScan^® Proteomic Services are applied in comparative studies to identify substrates and biomarkers of ubiquitin pathway targets and compounds.

UbiScan^® Workflow Diagram  0.4 MB

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Example Studies and Deliverables