| Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb #9634 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-(Ser/Thr) Phe Antibody #9631 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-(Ser) 14-3-3 Binding Motif Antibody #9601 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb #9606 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody #2981 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-4E-BP1 (Thr37/46) Antibody #9459 | 293 | Serum starve for 24 hrs, followed by a 1 hr amino acid depravation; then add back amino acids for 1 hr, and treat with 100 nM insulin for 30 min. | |
| Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb #2855 | 293 | Serum starve for 24 hrs, followed by a 1 hr amino acid depravation; then add back amino acids for 1 hr, and treat with 100 nM insulin for 30 min. | |
| Phospho-4E-BP1 (Ser65) Antibody #9451 | MEF, C2C12, C6 | Untreated | |
| Phospho-4E-BP1 (Ser65) (174A9) Rabbit mAb #9456 | 293 | Serum starve for 24 hrs, followed by a 1 hr amino acid depravation; then add back amino acids for 1 hr, and treat with 100 nM insulin for 30 min. | |
| Phospho-4E-BP1 (Thr70) Antibody #9455 | 293 | Serum starve for 24 hrs, followed by a 1 hr amino acid depravation; then add back amino acids for 1 hr, and treat with 100 nM insulin for 30 min. | |
| Phospho-53BP1 (Ser25/29) Antibody #2674 | HT-29 | UV2 (100 mJ/cm2) , 1 hr recovery before lysing | |
| Phospho-53BP1 (Ser1778) Antibody #2675 | HeLa | UV2 (100 mJ/cm2) , 1 hr recovery before lysing | |
| Phospho-c-Abl (Tyr245) Antibody #2861 | K562 | Untreated (K562 cell line contains Bcr-Abl fusion) | |
| Phospho-c-Abl (Tyr245) (73E5) Rabbit mAb #2868 | K562 | Untreated (K562 cell line contains Bcr-Abl fusion) | |
| Phospho-c-Abl (Tyr412) (247C7) Rabbit mAb #2865 | K562 | Untreated (K562 cell line contains Bcr-Abl fusion) | |
| Phospho-c-Abl (Thr735) Antibody #2864 | K562 | Untreated (K562 cell line contains Bcr-Abl fusion) | |
| Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody #3661 | C2C12 | Serum-starve overnight, AICAR (0.5 mM for 30 min) | AMPK Control Cell Extracts #9158 |
| Phospho-Ack1 (Tyr857/858) Antibody #3137 | Transfected1 | Transfect cells with construct overexpressing Ack1 | |
| Phospho-Akt (Thr308) Antibody #9275 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-Akt (Thr308) (244F9) Rabbit mAb #4056 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-Akt (Ser473) Antibody #9271 | Jurkat | Untreated or calyculin A (100 nM for 30 min); alternative: serum-starve, insulin (100nM for 10 min; works for most cell lines | Akt Control Cell Extracts #9273 |
| Phospho-Akt (Ser473) (193H12) Rabbit mAb #4058 | Jurkat | Untreated or calyculin A (100 nM for 30 min); alternative: serum-starve, insulin (100nM for 10 min; works for most cell lines | Akt Control Cell Extracts #9273 |
| Phospho-Akt (Ser473) (587F11) Mouse mAb #4051 | Jurkat | Untreated or calyculin A (100 nM for 30 min); alternative: serum-starve, insulin (100nM for 10 min; works for most cell lines | Akt Control Cell Extracts #9273 |
| Phospho-Drosophila Akt (Ser505) Antibody #3901 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-Akt (Thr450) Antibody #9267 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-(Ser/Thr) Akt Substrate Antibody #9611 | C2C12 | Insulin (100 nM for 15 min) | |
| Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb #9614 | C2C12 | Insulin (100 nM for 15 min) | |
| Phospho-ALK (Tyr1586) Antibody #3343 | Karpas 299 | Untreated (Karpas 299 cell lines contains NPM-ALK fusion) | |
| Phospho-ALK (Tyr1586) (3B4) Rabbit mAb #3348 | Karpas 299 | Untreated (Karpas 299 cell lines contains NPM-ALK fusion) | |
| Phospho-ALK (Tyr1604) Antibody #3341 | Karpas 299 | Untreated (Karpas 299 cell lines contains NPM-ALK fusion) | |
| Phospho-AML1 (Ser249) Antibody #4327 | HEL | Grow in serum | |
| Phospho-AMPKα (Thr172) Antibody #2531 | C2C12 | Serum-starve overnight, AICAR (0.5 mM for 30 min) | AMPK Control Cell Extracts #9158 |
| Phospho-AMPKα (Thr172) (40H9) Rabbit mAb #2535 | C2C12 | Serum-starve overnight, AICAR (0.5 mM for 30 min) | AMPK Control Cell Extracts #9158 |
| Phospho-AMPKα1 (Ser485) Antibody #4184 | C6 | Serum-starve overnight, oligomycin (0.5 μm for 30 min) | |
| Phospho-AMPKα1 (Ser485) (45F5) Rabbit mAb #2537 | 293 | Serum-starve overnight, AICAR (0.5 mM for 30 min) | |
| Phospho-AMPKα1 (Ser485)/AMPKα2 (Ser491) Antibody #4185 | C2C12 | Serum-starve overnight, AICAR (0.5 mM for 30 min) | AMPK Control Cell Extracts #9158 |
| Phospho-AMPKβ1 (Ser182) Antibody #4186 | C2C12 | Serum-starve overnight, AICAR (0.5 mM for 30 min) | AMPK Control Cell Extracts #9158 |
| Phospho-AMPKβ1 (Ser108) Antibody #4181 | C2C12 | Serum-starve overnight, AICAR (0.5 mM for 30 min) | AMPK Control Cell Extracts #9158 |
| Phospho-APP (Thr668) Antibody #2451 | C6, PC-12 | nocodazole (1 µg/ml for 18 hrs) | |
| Phospho-A-Raf (Ser299) Antibody #4431 | HeLa | Serum-starve overnight, TPA (200 nM for 15min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-β-Arrestin 1 (Ser412) (6-24) Mouse mAb #2416 | 293 | Untreated | |
| Phospho-ASK1 (Ser83) Antibody #3761 | Transfected1 | Transfect with construct overexpressing ASK1 | |
| Phospho-ASK1 (Thr845) Antibody #3765 | Transfected1 | Transfect with construct overexpressing ASK1 | |
| Phospho-ASK1(Ser967) Antibody #3764 | Transfected1 | Transfect with construct overexpressing ASK1 | |
| Phospho-ATF-2 (Thr69/71) Antibody #9225 | NIH/3T3 | Anisomycin (25 μg/ml for 30 min) | ATF-2 Control Cell Extracts #9223 |
| Phospho-ATF-2 (Thr71) Antibody #9221 | NIH/3T3 | Anisomycin (25 μg/ml for 30 min) | ATF-2 Control Cell Extracts #9223 |
| Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb #5112 | NIH/3T3 | Anisomycin (25 μg/ml for 30 min) | ATF-2 Control Cell Extracts #9223 |
| Phospho-ATM (Ser1981) (10H11.E12) Mouse mAb #4526 | 293 | IR (10 Gy), 1 hr recovery | |
| Phospho-(Ser/Thr) ATM/ATR Substrate Antibody #2851 | 293 | UV2 (50 mJ/cm2), 30 min recovery | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-ATP-Citrate Lyase (Ser454) Antibody #4331 | NIH/3T3 | Serum-starve overnight, PDGF (50 ng/ml for 10 min) | |
| Phospho-ATR (Ser428) Antibody #2853 | HeLa | UV2 (75 mJ/cm2) 1 hr recovery | |
| Phospho-Bad (Ser112) Antibody #9291 | COS | Serum-starve overnight, TPA (200 nM for 30 min) | 9293 |
| Phospho-Bad (Ser112) (40A9) Rabbit mAb #5284 | COS | Serum-starve overnight, TPA (200 nM for 30 min) | 9293 |
| Phospho-Bad (Ser112) (7E11) Mouse mAb #9296 | COS | Serum-starve overnight,TPA (200 nM for 30 min) | 9293 |
| Phospho-Bad (Ser136) Antibody #9295 | Transfected1 | Serum-starve overnight, PDGF (100 ng/ml for 30 min) | 9293 |
| Phospho-Bad (Ser155) Antibody #9297 | Transfected1 | Serum-starve overnight, PDGF (100 ng/ml for 30 min) | 9293 |
| Phospho-Bcl-2 (Thr56) Antibody (Human Specific) #2875 | Jurkat | Serum-starve overnight, paclitaxel (1 μM for 18 hrs) | |
| Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb #2827 | Jurkat | Serum-starve overnight, paclitaxel (1 μM for 18 hrs) | |
| Phospho-Bcr (Tyr177) Antibody #3901 | K562 | Untreated (K562 cell line contains Bcr-Abl fusion) | |
| Phospho-BLNK (Tyr96) Antibody #3601 | Ramos | Anti-IgM (12 µg/ml for 2 min) | |
| Phospho-B-Raf (Ser445) Antibody #2696 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-BRCA1 (Ser1524) Antibody #9009 | HeLa | UV2 (75 mJ/cm2), 1 hr recovery | |
| Phospho-Btk (Ser180) (3D3) Mouse mAb #3537 | THP1 | Serum-starve overnight, H2O2 (2mM for 2 min) | |
| Phospho-C/EBPα (Ser21) Antibody #2841 | differentiated 3T3-L1 | Serum-starve overnight, insulin (100 nM for 45 min) | |
| Phospho-C/EBPα (Thr222/226) Antibody #2844 | U937 | Untreated | |
| Phospho-C/EBPβ (Ser105) Antibody (Rat Specific) #3081 | PC12 | Untreated | |
| Phospho-C/EBPβ (Thr235) Antibody #3084 | differentiated 3T3-L1 | Serum-starve overnight, insulin (100 nM for 10 min) | |
| Phospho-CaMKII (Thr286) Antibody #3361 | HeLa | TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Cleaved Caspase-3 (Asp175) (5A1) Rabbit mAb #9664 | HeLa | Serum-starve overnight, staurosporine (1 μM for 3 hrs) | Caspase-3 Control Cell Extracts #9663 |
| Cleaved Caspase-3 (Asp175) Antibody #9661 | HeLa | Serum-starve overnight, staurosporine (1 μM for 3 hrs) | Caspase-3 Control Cell Extracts #9663 |
| Cleaved Caspase-6 (Asp162) Antibody #9761 | HeLa | Serum-starve overnight, staurosporine (1 μM for 3 hrs) | Caspase-3 Control Cell Extracts #9663 |
| Cleaved Caspase-7 (Asp198) Antibody #9491 | HeLa | Serum-starve overnight, staurosporine (1 μM for 3 hrs) | Caspase-3 Control Cell Extracts #9663 |
| Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb #9496 | Jurkat | Etoposide (25 μM for 5 hrs) | Jurkat Apoptosis Cell Lysates (etoposide) #2043 |
| Cleaved Caspase-8 (Asp384) (11G10) Mouse mAb #9748 | Jurkat | Etoposide (25 μM for 5 hrs) | Jurkat Apoptosis Cell Lysates (etoposide) #2043 |
| Phospho-Caspase-9 (Thr125) Antibody #2226 | MCF-7 | EGF (10 ng/mL for 30 min) | |
| Cleaved Caspase-9 (Asp315) Antibody (Human Specific) #9505 | Jurkat | Etoposide (25 μM for 5 hrs) | Jurkat Apoptosis Cell Lysates (etoposide) #2043,
Caspase-3 Control Cell Extracts #9663 |
| Cleaved Caspase-9 (Asp330) Antibody (Human Specific) #9501 | Jurkat | Etoposide (25 μM for 5 hrs) | Jurkat Apoptosis Cell Lysates (etoposide) #2043,
Caspase-3 Control Cell Extracts #9663 |
| Cleaved Caspase-9 (Asp353) Antibody (Mouse Specific) #9509 | L929 | Serum-starve overnight, staurosporine (1 µM for 3 hrs) | |
| Cleaved Caspase-9 (Asp353) Antibody (Rat Specific) #9507 | C6 | Serum-starve overnight, staurosporine (1 µM for 3 hrs) | |
| Phospho-β-Catenin (Ser33/37/Thr41) Antibody #9561 | sw480 | Untreated | |
| Phospho-β-Catenin (Ser45) Antibody #9564 | sw480 | Untreated | |
| Phospho-β-Catenin (Thr41/Ser45) Antibody #9565 | sw480 | Untreated | |
| Acetyl-β-Catenin (Lys49) Antibody #9534 | Transfected1 | Transfect with a construct overexpressing CBP | |
| Phospho-Caveolin-1 (Tyr14) Antibody #3251 | HeLa | Serum-starve overnight, H2O2 (500 μM for 15 min) | |
| Phospho-c-Cbl (Tyr731) Antibody #3554 | K562 | Untreated | |
| Phospho-c-Cbl (Tyr774) Antibody #3555 | K562 | Untreated | |
| Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody #4771 | NIH/3T3 | TSA (400 nM overnight) | |
| Phospho-CD19 (Tyr513) Antibody #3571 | Ramos | Anti-IgM (12 μg/ml for 2 min) | |
| Phospho-cdc2 (Thr14) Antibody #2543 | HeLa | Nocodazole (50 ng/mL for 20 hrs) | |
| Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb #4539 | HeLa | Untreated | cdc2 (Tyr15) Control Proteins #9113 |
| Phospho-cdc2 (Tyr15) Antibody #9111 | HeLa | Untreated | cdc2 (Tyr15) Control Proteins #9113 |
| Phospho-cdc2 (Thr161) Antibody #9114 | HeLa | Nocodazole (50 ng/mL for 20 hrs) | |
| Phospho-cdc25C (Ser198) Antibody #9529 | Transfected1 | Transfect with construct overexpressing cdc25C; Nocodazole (100 ng/ml for 18 hrs)- treatment induces signal | |
| Phospho-cdc25C (Ser216) (63F9) Rabbit mAb #4901 | HT-29 | Nocodazole (100 ng/ml for 18 hrs) - treatment induces signal | |
| Phospho-cdc25C (Ser216) Antibody #9528 | HT-29 | Nocodazole (100 ng/ml for 18 hrs) - treatment diminishes signal | |
| Phospho-cdc25C (Thr48) Antibody #9527 | HT-29 | Nocodazole (100 ng/ml for 18 hrs) - treatment induces signal | |
| Phospho-CDK2 (Thr160) Antibody #2561 | HeLa | Nocodazole (50 ng/mL for 20 hrs) | |
| Phospho-(Ser) CDKs Substrate Antibody #2324 | HeLa | Nocadazole (1 μg/ml for 12 hrs) | |
| Phospho-Chk1(Ser280) Antibody #2347 | HeLa | UV2(75 mJ/cm2), 1 hr recovery | |
| Phospho-Chk1 (Ser296) Antibody #2349 | 293 | UV2 (100 mJ/cm2), 1 hr recovery | |
| Phospho-Chk1 (Ser317) Antibody #2344 | HeLa | UV2 (100 mJ/cm2), 1 hr recovery | |
| Phospho-Chk1 (Ser345) Antibody #2341 | HeLa | UV2 (100 mJ/cm2), 1 hr recovery | |
| Phospho-Chk1 (Ser345) (133D3) Rabbit mAb #2348 | HeLa | UV2 (75 mJ/cm2), 1 hr recovery | |
| Phospho-Chk2 (Ser19) Antibody #2666 | HeLa | UV2 (100 mJ/cm2), 1 hr recovery | |
| Phospho-Chk2 (Ser33/35) Antibody #2665 | HeLa | UV2 (75 mJ/cm2), 1 hr recovery | |
| Phospho-Chk2 (Ser516) Antibody #2669 | HeLa | UV2 (100 mJ/cm2), 1 hr recovery | |
| Phospho-Chk2 (Thr387) Antibody #2668 | 293 | UV2 (100 mJ/cm2), 1 hr recovery | |
| Phospho-Chk2 (Thr432) Antibody #2667 | HeLa | UV2 (100 mJ/cm2), 1 hr recovery | |
| Phospho-Chk2 (Thr68) Antibody #2661 | HeLa | IR (10 Gy), 30 min recovery | |
| Phospho-Cofilin (Ser3) (77G2) Rabbit mAb #3313 | C2C12, NIH/3T3, HeLa | Untreated | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-Cofilin (Ser3) Antibody #3311 | C2C12, NIH/3T3, HeLa | Untreated | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-Connexin 43 (Ser368) Antibody #3511 | COS | Serum-starve overnight, PMA (200 nM for 30 min) | |
| Phospho-cPLA2 (Ser505) Antibody #2831 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160,
NF-κB Control Cell Extracts #9243 |
| Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198 | SK-N-MC | Forskolin (30 µM) and IBMX (0.5 mM), for 30 min | CREB Control Cell Extracts #9193 |
| Phospho-CREB (Ser133) (1B6) Mouse mAb #9196 | SK-N-MC | Forskolin (30 µM) and IBMX (0.5 mM), for 30 min | CREB Control Cell Extracts #9193 |
| Phospho-CREB (Ser133) Antibody #9191 | SK-N-MC | Forskolin (30 µM) and IBMX (0.5 mM), for 30 min | CREB Control Cell Extracts #9193 |
| Phospho-CrkII (Tyr221) Antibody #3491 | K562 | Untreated | |
| Phospho-CrkL (Tyr207) Antibody #3181 | K562 | Untreated | |
| Phospho-M-CSF Receptor (Tyr723) Antibody #3151 | NKM1 | Untreated | |
| Phospho-M-CSF Receptor (Tyr723) (49C10) Rabbit mAb #3155 | NKM1 | Untreated | |
| Phospho-M-CSF Receptor (Tyr809) Antibody #3154 | NKM1 | Untreated | |
| Phospho-Cyclin B1 (Ser147) Antibody #4131 | HeLa | Nocodazole (50 ng/mL for 20 hrs) | |
| Phospho-Cyclin D1 (Thr286) Antibody #2921 | Mcf-7 | Hydroxyurea (10 mM for 8 hrs) | |
| Phospho-Cyclin E (Thr62) Antibody #4136 | Mcf-7 | Hydroxyurea (10 mM for 8 hrs) | |
| Phospho-Dab1 (Tyr220) Antibody #3327 | Transfected1 | Cotransfect with Dab1 and fyn expression contructs | |
| Phospho-Dab1 (Tyr232) Antibody #3325 | Transfected1 | Cotransfect with Dab1 and fyn expression contructs | |
| Phospho-DARPP-32 (Thr34) Antibody #2304 | Mouse brain/rat brain | PKA (12,500 U/100 μl for 1 hr) | |
| Phospho-DARPP-32 (Thr75) Antibody #2301 | Mouse brain/rat brain | PKA (12,500 U/100 μl for 1 hr) | |
| Phospho-Doublecortin (Ser297) Antibody #4605 | Fetal rat brain | Untreated | |
| Cleaved DFF45 (Asp224) Antibody #9731 | Jurkat | Etoposide (25 µM for 5 hrs) | Jurkat Apoptosis Cell Lysates (etoposide) #2043,
Caspase-3 Control Cell Extracts #9663 |
| Phospho-eEF2 (Thr56) Antibody #2331 | C6 | Forskolin (10 µM for 60 min) | |
| Phospho-eEF2k (Ser366) Antibody #3691 | HeLa, NIH/3T3, C6, COS | Untreated | |
| Phospho-EGF Receptor (Tyr845) Antibody #2231 | HT29 | Serum-starve overnight, EGF (100 ng/ml for 5 min) | |
| Phospho-EGF Receptor (Tyr992) Antibody #2235 | HT29 | Serum-starve overnight, EGF (100 ng/ml for 5 min) | |
| Phospho-EGF Receptor (Tyr1045) Antibody #2237 | HT29 | Serum-starve overnight, EGF (100 ng/ml for 5 min) | |
| Phospho-EGF Receptor (Ser1046/1047) Antibody #2238 | HT29 | Serum-starve overnight, EGF (100 ng/ml for 5 min) | |
| Phospho-EGF Receptor (Tyr1068) Antibody #2234 | HT29 | Serum-starve overnight, EGF (100 ng/ml for 5 min) | |
| Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb #2236 | HT29 | Serum-starve overnight, EGF (100 ng/ml for 5 min) | |
| Phospho-EGF Receptor (Tyr1086) Antibody #2220 | HT29 | Serum-starve overnight, EGF (100 ng/ml for 5 min) | |
| Phospho-EGF Receptor (Tyr1148) Antibody #4404 | HT29 | Serum-starve overnight, EGF (100 ng/ml for 5 min) | |
| Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb #4407 | HT29 | Serum-starve overnight, EGF (100 ng/ml for 5 min) | |
| Phospho-eIF2α (Ser51) Antibody #9721 | C6 | Thapsigargin (300 nM for 30 min) | |
| Phospho-eIF2α (Ser51) (119A11) Rabbit mAb #3597 | C6 | Thapsigargin (300 nM for 30 min) | |
| Phospho-eIF4E (Ser209) Antibody #9741 | NIH/3T3 | Serum starve overnight, 20% serum for 20 min | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-eIF4B (Ser422) Antibody #3591 | HeLa, COS, NIH/3T3, PC-12 | Untreated | |
| Phospho-eIF4G (Ser1108) Antibody #2441 | PC12 | Untreated | |
| Phospho-eNOS (Ser113) Antibody #9575 | BAEC | Serum-starve overnight, VEGF (50 ng/ml for 5 min) | |
| Phospho-eNOS (Thr495) Antibody #9574 | BAEC | Serum-starve overnight, VEGF (50 ng/ml for 5 min) | |
| Phospho-eNOS (Ser1177) Antibody #9571 | BAEC | Serum-starve overnight,VEGF (50 ng/ml for 5 min) | |
| Phospho-eNOS (Ser1177) (C9C3) Rabbit mAb #9570 | BAEC | Serum-starve overnight, VEGF (50 ng/ml for 5 min) | |
| Phospho-Ephrin B (Tyr324/329) Antibody #3481 | Transfected1 | NIH/3T3 cells stably expressing Ephrin B1, stimulated with its cognate receptor EphB2-Fc | |
| Phospho-Erk5 (Thr218/Tyr220) Antibody #3371 | NIH/3T3 | Serum-starve overnight, PDGF (100 ng/ml for 5 min); IP with #3372 prior to Western | |
| Phospho-Estrogen Receptor α (Ser104/106) Antibody #2517 | MCF-7 | Serum-starve overnight, EGF (100 ng/ml) plus estradiol (100 nM) for 30 min | |
| Phospho-Estrogen Receptor α (Ser118) Antibody #2515 | MCF-7 | Serum-starve overnight, EGF (100 ng/ml) plus estradiol (100 nM) for 30 min | |
| Phospho-Estrogen Receptor α (Ser118) (16J4) Mouse mAb #2511 | MCF-7 | Serum-starve overnight, EGF (100 ng/ml) plus estradiol (100 nM) for 30 min | |
| Phospho-Estrogen Receptor α (Ser167) Antibody #2514 | Transfected1 | Transfect COS cells with construct overexpressing estrogen receptor | |
| Phospho-Etk (Tyr40) Antibody #3211 | Jurkat | Serum-starve overnight, anti-CD3 (1 μg/ml) plus anti-CD28 (0.5 μg/ml) for 10 min | |
| Phospho-Ezrin (Tyr353) Antibody #3144 | A431 | Serum-starve overnight, EGF (100 ng/ml for 10 min) | |
| Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody #3141 | C2C12, NIH/3T3, HeLa | Untreated | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (41A3) Rabbit mAb #3149 | C2C12, NIH/3T3, HeLa | Untreated | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-FADD (Ser191) Antibody (Mouse Specific) #2785 | L-929 | Inhibit with hydroxyurea (4.0 mM for 24 hrs); activate with nocodazole (1.0 μg/mL) for 24 hrs | |
| Phospho-FADD (Ser194) Antibody (Human Specific) #2781 | Jurkat | Inhibit with hydroxyurea (4.0 mM for 24 hrs); activate with nocodazole (1.0 μg/mL) for 24 hrs | |
| Phospho-FAK (Tyr576/577) Antibody #3281 | A549 | Untreated | |
| Phospho-FAK (Tyr925) Antibody #3284 | A549 | Untreated | |
| Phospho-FANCD2 (Ser222) Antibody #4945 | HeLa | UV2 (100 mJ/cm2), 1 hr recovery | |
| Phospho-CD32 (FcγRIIb) (Tyr292) Antibody #4141 | THP1 | CD32 (10 μg/ml for 30 min), then α-mouse IgG (18 μg/ml for 5 min) | |
| Phospho-FGF Receptor (Tyr653/654) Antibody #3471 | Transfected1 | Transfect with a construct overexpressing FGFR1 | |
| Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse mAb #3476 | Transfected1 | Transfect with a construct overexpressing FGFR1 | |
| Phospho-FGF Receptor 1 (Tyr766) (1E5) Rabbit mAb #2544 | Transfected1 | Transfect with a construct overexpressing FGFR1 | |
| Phospho-Filamin A (Ser2152) Antibody #4761 | 293 | Untreated | |
| Phospho-FLT3 (Tyr589/591) (30D4) Rabbit mAb #3464 | MV4-11 | Untreated | |
| Phospho-FLT3 (Tyr591) Antibody #3461 | MV4-11 | Untreated | |
| Phospho-FLT3 (Tyr591) (33G6) Rabbit mAb #3474 | MV4-11 | Untreated | |
| Phospho-FLT3 (Tyr591) (54H1) Mouse mAb #3466 | MV4-11 | Untreated | |
| Phospho-FLT3 (Tyr969) (C24D9) Rabbit mAb #3463 | MV4-11 | Untreated | |
| Phospho-FoxO1 (Thr24)/FoxO3a (Thr32) Antibody #9464 | HeLa | Serum-starve overnight, 20% serum for 30 min | |
| Phospho-FoxO1 (Ser256) Antibody #9461 | HeLa | Serum-starve overnight, 20% serum for 30 min | |
| Phospho-FoxO1 (Ser319) Antibody #2486 | HeLa | Serum-starve overnight, 20% serum for 30 min | |
| Phospho-FoxO1 (Ser319)/FoxO4 (Ser262) Antibody #2487 | HeLa | Serum-starve overnight, 20% serum for 30 min | |
| Phospho-FoxO3a (Ser253) Antibody #9466 | HeLa | Serum-starve overnight, 20% serum for 30 min | |
| Phospho-FoxO3a (Ser318/321) Antibody #9465 | HeLa | Serum-starve overnight, 20% serum for 30 min | |
| Phospho-FoxO4 (Ser193) Antibody #9471 | HeLa | Serum-starve overnight, 20% serum for 30 min | |
| Phospho-FRS2-α (Tyr196) Antibody #3864 | NIH/3T3 | Serum-starve overnight, bFGF (100 ng/ml for 10 min) | |
| Phospho-FRS2-α (Tyr436) Antibody #3861 | NIH/3T3 | Serum-starve overnight, bFGF (100 ng/ml for 10 min) | |
| Phospho-Gab1 (Tyr307) Antibody #3234 | HepG2 | Serum-starve overnight, HGF (40 ng/ml for 10 min) | |
| Phospho-Gab1 (Tyr627) Antibody #3231 | H4IIE | Serum-starve overnight, HGF (40 ng/ml for 10 min) | |
| Phospho-Gab2 (Ser159) Antibody #3884 | H4IIE | Serum-starve overnight, HGF (40 ng/ml for 10 min) | |
| Phospho-Gab2 (Tyr452) Antibody #3881 | H4IIE | Serum-starve overnight, HGF (40 ng/ml for 10 min) | |
| Phospho-GCN2 (Thr898) Antibody #3301 | 293, C6 | UV2 (50 mJ/cm2), 30 min recovery | |
| Phospho-Glucocorticoid Receptor (Ser211) Antibody #4161 | A549 | Serum-starve overnight, dexamethasone (100 nM for 1 hr) | |
| Phospho-Glycogen Synthase (Ser641) Antibody #3891 | 293 | Serum-starve overnight, insulin (100 nM for 5 min) | |
| Phospho-GRB10 (Tyr67) Antibody #3701 | H4IIE | Serum-starve overnight, HGF (40 ng/ml for10 min) | |
| Phospho-GSK-3β (Ser9) Antibody #9336 | COS | Serum-starve overnight, PDGF (100 ng/ml for 30 min) | |
| Phospho-GSK-3α/β (Ser21/9) Antibody #9331 | COS | Serum-starve overnight, PDGF (100 ng/ml for 30 min) | |
| Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb #9316 | COS | Serum-starve overnight, PDGF (100 ng/ml for 30 min) | |
| Phospho-GSK-3α (Ser21) (46H12) Mouse mAb #9337 | COS | Serum-starve overnight, PDGF (100 ng/ml for 30 min) | |
| Phospho-GSK-3α/β (Ser21/9) (37F11) Rabbit mAb (GSK-3α Preferred) #9327 | COS | Serum-starve overnight, PDGF (100 ng/ml for 30 min) | |
| Phospho-HER2/ErbB2 (Tyr877) Antibody #2241 | MCF-7 | Serum-starve overnight, neuregulin (100 ng/ml for 5 min) | |
| Phospho-HER2/ErbB2 (Tyr1221/1222) Antibody #2249 | MCF-7 | Serum-starve overnight, neuregulin (100 ng/ml for 5 min) | |
| Phospho-HER2/ErbB2 (Tyr1221/1222) (6B12) Rabbit mAb #2243 | MCF-7 | Serum-starve overnight, neuregulin (100 ng/ml for 5 min) | |
| Phospho-HER3/ErbB3 (Tyr1222) (50C2) Rabbit mAb #4784 | MCF-7 | Serum-starve overnight, neuregulin (100 ng/ml for 5 min) | |
| Phospho-HER2/ErbB2(Tyr1248) Antibody #2247 | MCF-7 | Serum-starve overnight, neuregulin (100 ng/ml for 5 min) | |
| Phospho-HER2/ErbB2 (Tyr1248)/EGFR (Tyr1173) Antibody #2244 | MCF-7 | Serum-starve overnight, neuregulin (100 ng/ml for 5 min) | |
| Phospho-HER4/ErbB4 (Tyr1284) (21A9) Rabbit mAb #4757 | MCF-7 | Serum-starve overnight, neuregulin (100 ng/ml for 5 min) | |
| Phospho-HER3/ErbB3 (Tyr1289) (21D3) Rabbit mAb #4791 | MCF-7 | Serum-starve overnight, neuregulin (100 ng/ml for 5 min) | |
| Acetyl-Histone H2A (Lys5) Antibody #2576 | NIH/3T3 | TSA (400 nM overnight) | |
| Phospho-Histone H2A.X (Ser139) Antibody #2577 | 293 | UV2 (40 mJ/cm2), 2 hr recovery | |
| Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718 | 293 | UV2 (40 mJ/cm2), 2 hr recovery | |
| Acetyl-Histone H2B (Lys5) Antibody #2574 | NIH/3T3 | TSA (400 nM overnight) | |
| Acetyl-Histone H2B (Lys12) Antibody #2575 | NIH/3T3 | TSA (400 nM overnight) | |
| Acetyl-Histone H2B (Lys20) Antibody #2571 | NIH/3T3 | TSA (400 nM overnight) | |
| Methyl-Histone H3 (Arg2) Antibody #9707 | human | Untreated | |
| Acetyl-Histone H3 (Lys9) Antibody #9671 | NIH/3T3 | TSA (400 nM overnight) | |
| Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb #9649 | NIH/3T3 | TSA (400 nM overnight) | |
| Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Antibody #9711 | NIH/3T3 | Serum-starve plus 400 nM TSA overnight, add 20% serum (15 min 20%), then 100 nM Calyculin A for 15 min | |
| Acetyl-Histone H3 (Lys9/Lys14) Antibody #9677 | NIH/3T3 | TSA (400 nM overnight) | |
| Acetyl-Histone H3 (Lys18) Antibody #9675 | NIH/3T3 | TSA (400 nM overnight) | |
| Acetyl-Histone H3 (Lys23) Antibody #9674 | NIH/3T3 | TSA (400 nM overnight) | |
| Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb #9725 | NIH/3T3 | Untreated | |
| Di-Methyl-Histone H3 (Lys27) Antibody #9755 | NIH/3T3 | Untreated | |
| Di-Methyl-Histone H3 (Lys36) Antibody #9758 | NIH/3T3 | Untreated | |
| Di-Methyl-Histone H3 (Lys79) Antibody #9757 | NIH/3T3 | Untreated | |
| Tri-Methyl-Histone H3 (Lys4) Antibody #9727 | NIH/3T3 | Untreated | |
| Di-Methyl-Histone H3 (Lys9) Antibody #9753 | NIH/3T3 | Untreated | |
| Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 | NIH/3T3 | Serum-starve overnight, add 20% serum (15 min), then Calyculin A (100 nM for 15 min) | |
| Phospho-Histone H3 (Ser10) Antibody #9701 | NIH/3T3 | Serum-starve overnight, add 20% serum (15 min), then Calyculin A (100 nM for 15 min) | |
| Phospho-Histone H3 (Ser28) Antibody #9713 | HeLa | Serum-starve overnight, add 20% serum (15 min), then Calyculin A (100 nM for 15 min) | |
| Phospho-Histone H3 (Thr11) Antibody #9764 | NIH/3T3 | Serum-starve overnight, add 20% serum (15 min), then Calyculin A (100 nM for 15 min) | |
| Phospho-Histone H3 (Thr3) Antibody #9714 | NIH/3T3 | Serum-starve overnight, add 20% serum (15 min), then Calyculin A (100 nM for 15 min) | |
| Acetyl-Histone H4 (Lys8) Antibody #2594 | NIH/3T3 | TSA (400 nM, overnight) | |
| Phospho-HSL (Ser563) Antibody #4139 | differentiated 3T3-L1 | Isoproterenol (10 µM for 20 min) | |
| Phospho-HSL (Ser565) Antibody #4137 | differentiated 3T3-L1 | Isoproterenol (10 µM for 20 min) | |
| Phospho-HSL (Ser660) Antibody #4126 | differentiated 3T3-L1 | Isoproterenol (10 µM for 20 min) | |
| Phospho-HSP27 (Ser15) Antibody #2404 | HeLa | Serum-starve overnight, UV (40 mJ/cm2), 20 min recovery | |
| Phospho-HSP27 (Ser78) Antibody #2405 | HeLa | Serum-starve overnight, UV (40 mJ/cm2), 20 min recovery | |
| Phospho-HSP27 (Ser82) Antibody #2401 | HeLa | Serum-starve overnight, UV (40 mJ/cm2), 20 min recovery | |
| Phospho-IGF-I Receptor β (Tyr1131)/Insulin Receptor β (Tyr1146) Antibody #3021 | H4IIE | Serum-starve overnight, insulin (100 nM for 5 min) | |
| Phospho-IGF-I Receptor β (Tyr1135/1136)/Insulin Receptor β (Tyr1150/1151) (19H7) Rabbit mAb #3024 | MCF7 | Serum-starve overnight, insulin (100 nM for 5 min) | |
| Phospho-IκB-α (Ser32) (14D4) Rabbit mAb #2859 | HeLa | TNF-α (20 ng/ml for 5 min) | NF-κB Control Cell Extracts #9243 |
| Phospho-IκB-α (Ser32/36) (5A5) Mouse mAb #9246 | HeLa | TNF-α (20 ng/ml for 5 min) | NF-κB Control Cell Extracts #9243 |
| Phospho-IκB-β (Thr19/Ser23) Antibody (Human Specific) #4921 | HeLa | TNF-α (20 ng/ml) plus Calyculin A (50 nM) for 5 min | |
| Phospho-IκBβ (Ser19/23) Antibody (Mouse/Rat Specific) #9245 | RAW264.7 | LPS (1 μg/ml for 15 min) | |
| Phospho-IκB-ε (Ser18/22) Antibody #4924 | HeLa | TNF-α (20 ng/ml) plus Calyculin A (50 nM) for 5 min | |
| Phospho-IKKα/β (Ser176/180) Antibody #2687 | HeLa | TNF-α (20 ng/ml for 10 min) | NF-κB Control Cell Extracts #9243 |
| Phospho-IKKα/β (Ser176/180) Antibody II #2694 | HeLa | TNF-α (20 ng/ml for 10 min) | NF-κB Control Cell Extracts #9243 |
| Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb #2697 | 293 | TNF-α (20 ng/ml for 10 min) | |
| Phospho-IKKα (Ser180)/IKKβ (Ser181) Antibody #2681 | NIH/3T3 | TNF-α (20 ng/ml for 10 min) | |
| Phospho-IKKγ (Ser376) Antibody #2689 | HeLa | TNF-α (20 ng/ml for 10 min) | NF-κB Control Cell Extracts #9243 |
| Cleaved IL-1β (Asp116) Antibody #2021 | THP-1 | LPS (10 µg/ml for 24 hrs), then TPA (0.5 µM for 3 hrs) | |
| Phospho-Insulin Receptor β (Tyr1345) (14A4) Rabbit mAb #3026 | Transfected1 | Transfect with IR/IRS-1, serum-starve overnight, insulin (100 nM for 5 min) | |
| Phospho-Insulin Receptor β (Tyr1361) (84B2) Rabbit mAb #3023 | Transfected1 | Transfect with IR/IRS-1, serum-starve overnight, insulin (100 nM for 5 min) | |
| Phospho-IRAK1 (Ser376) Antibody #4361 | HeLa | IL-1β (20 ng/ml) plus Calyculin A (50 nM) for 5 min | |
| Phospho-IRF-3 (Ser396) Antibody #4961 | HT29 | DEAE-Dextran 1:20/polyI-polyC 100 µg/ml transfection, assay after 1 hr | |
| Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb #4947 | THP-1 | Differentiate with TPA (80 nM for 24 hours), rest for 24 hours, then treat with LPS (1 ug/ml for 1 hour) | |
| Phospho-IRS-1 (Ser302) Antibody #2384 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5 min) | |
| Phospho-IRS-1 (Ser307) Antibody #2381 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5 min) | |
| Phospho-IRS-1 (Ser307) (34C7) Rabbit mAb #2491 | Transfected1 | Transfect with IR/IRS-1, serum-starve overnight, insulin (100 nM for 15 min) | |
| Phospho-IRS-1 (Ser612) Antibody #2386 | mIMCD3 | Serum-starve overnight, insulin (100 nM for 5 min) | |
| Phospho-IRS-1 (Ser612) (L7B8) Mouse mAb #3193 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5 min) | |
| Phospho-IRS-1 (Ser636/639) Antibody #2388 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5 min) | |
| Phospho-IRS-1 (Ser789) Antibody #2389 | Transfected1 | Transfect with IR/IRS-1, serum-starve overnight, insulin (100nM for 10 min) | |
| Phospho-IRS-1 (Ser1101) Antibody #2385 | Differentiated C2C12 (myotubes) | Serum-starve overnight, insulin (100 nM for 5 min) | |
| Phospho-Jak1 (Tyr1022/1023) Antibody #3331 | HT-29 | Serum-starve overnight, IL-4 (100 ng/ml for 5-10 min) | |
| Phospho-Jak2 (Tyr221) Antibody #3774 | CTLL2 | Untreated | |
| Phospho-Jak2 (Tyr1007/1008) Antibody #3771 | TF-1 | Serum-starve overnight; GM-CSF (2 ng/ml) should be included during growth and serum-starvation | |
| Phospho-c-Jun (Ser63) II Antibody #9261 | NIH/3T3 | UV2 (40 mJ/cm2), 30 min recovery time | c-Jun Control Cell Extracts #9263 |
| Phospho-c-Jun (Ser63) (54B3) Rabbit mAb #2361 | NIH/3T3 | UV2 (40 mJ/cm2), 30 min recovery time | c-Jun Control Cell Extracts #9263 |
| Phospho-c-Jun (Ser73) Antibody #9164 | NIH/3T3 | UV2 (40 mJ/cm2), 30 min recovery time | c-Jun Control Cell Extracts #9263 |
| Phospho-c-Jun (Thr91) Antibody #2303 | NIH/3T3 | UV2 (40 mJ/cm2), 30 min recovery time | c-Jun Control Cell Extracts #9263 |
| Phospho-c-Jun (Thr93) Antibody #2993 | NIH/3T3 | constitutively phosphorylated | c-Jun Control Cell Extracts #9263 |
| Phospho-c-Kit (Tyr719) Antibody #3391 | H526 | Serum-starve overnight, stem cell factor (100 ng/ml for 5 min) | |
| Phospho-KSR1 (Ser392) Antibody #4951 | HeLa | Untreated; site is constitutively phosphorylated | |
| Cleaved Lamin A (Asp230) Antibody #2031 | HeLa | Serum-starve overnight, staurosporine (1.0 μM for 4 hrs) | |
| Cleaved Lamin A (Small Subunit) (30H5) Mouse mAb #2036 | HeLa | Serum-starve overnight, staurosporine (1.0 μM for 4 hrs) | |
| Cleaved Lamin A (Small Subunit) Antibody #2035 | HeLa | Serum-starve overnight, staurosporine (1.0 μM for 4 hrs) | |
| Phospho-LAT (Tyr171) Antibody #3581 | Jurkat | Anti-CD3 (10 μg/ml for 2 min) | |
| Phospho-LAT (Tyr191) Antibody #3584 | Jurkat | Anti-CD3 (10 μg/ml for 2 min) | |
| Phospho-Lck (Tyr505) Antibody #2751 | Jurkat | Serum-starve overnight, H2O2 (2mM for 2 min) | |
| Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody #3841 | Transfected1 | Transfect with construct overexpressing LIMK1 | |
| Phospho-LKB1 (Thr189) Antibody #3054 | Transfected1 | Transfect with construct overexpressing LKB1 | |
| Phospho-LKB1 (Ser334) Antibody #3055 | Transfected1 | Transfect with construct overexpressing LKB1 | |
| Phospho-LKB1 (Ser428) Antibody #3051 | Transfected1 | Transfect with construct overexpressing LKB1 | |
| Phospho-LRP6 (Ser1490) Antibody #2568 | HeLa | Wnt3a-conditioned media for 5 hrs (Conditioned media from L cells transfected with Wnt3a ligand) | |
| Phospho-Lyn (Tyr507) Antibody #2731 | Ramos | Serum-starve overnight, anti-IgM (12 μg/ml for 2 min) | |
| Acetylated-Lysine Antibody #9441 | COS | TSA 0.4 μM for 18 hr | |
| Acetylated-Lysine Mouse mAb (Ac-K-103) #9681 | COS | TSA 0.4 μM for 18 hr | |
| Phospho-MAP2 (Ser136) Antibody #4541 | PC-12 | Positive control: nocodazole (1µg/ml for 16 hrs); negative control: λ phosphatase (800 U/500 μl for 1 hr) | |
| Phospho-MAP2 (Thr1620/1623) Antibody #4544 | PC-12 | Positive control: nocodazole (1µg/ml for 16 hrs); negative control: λ phosphatase (800 U/500 μl for 1 hr) | |
| Phospho-(Thr) MAPK/CDK Substrate Mouse mAb #2321 | Jurkat | Nocadazole (1 μg/ml for 12 hr) | |
| Phospho-MAPK Substrates (PXTP) (46G11) Rabbit mAb #4391 | HeLa | Nocadazole (1 μg/ml for 12 hr) | |
| Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb #2325 | HeLa | Nocadazole (1 μg/ml for 12 hr) | |
| Phospho-MAPKAPK-2 (Thr222) Antibody #3044 | HeLa | Serum-starve overnight, UV (40 mJ/cm2), 20 min recovery | |
| Phospho-MAPKAPK-2 (Thr334) Antibody #3041 | HeLa | Serum-starve overnight, UV (40 mJ/cm2), 20 min recovery | |
| Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb #3007 | HeLa | Serum-starve overnight, UV (20 min at 40 mJ/cm2), 20 min recovery | |
| Phospho-MARCKS (Ser152/156) Antibody #2741 | HeLa, NIH/3T3 | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-MARK Family (Activation Loop) Antibody #4836 | Raji, BaF3 | Untreated | |
| Phospho-MDM2 (Ser166) Antibody #3521 | MCF-7 | IGF-1 (100 ng/ml for 15min) | |
| Phospho-MEF2A (Ser408) Antibody #9737 | Transfected1 | Transfect with construct overexpressing MEF2A | |
| Phospho-MEK1 (Thr286) Antibody #9127 | HeLa | Serum-starve overnight, nocodazole (0.2 μg/ml for 16 hrs) | |
| Phospho-MEK1 (Ser298) Antibody #9128 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-MEK1/2 (Ser217/221) Antibody #9121 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb #9154 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb #2338 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-Met (Tyr1234/1235) Antibody #3126 | H4IIE | Serum-starve overnight, HGF (40 ng/ml for 10 min) | |
| Phospho-Met (Tyr1234/1235) (3D7) Rabbit mAb #3129 | H4IIE | Serum-starve overnight, HGF (40 ng/ml for 10 min) | |
| Phospho-Met (Tyr1349) Antibody #3121 | H4IIE | Serum-starve overnight, HGF (40 ng/ml for 10 min) | |
| Phospho-Met (Tyr1349) (130H2) Rabbit mAb #3133 | H4IIE | Serum-starve overnight, HGF (40 ng/ml for 10 min) | |
| Phospho-MKK3/MKK6 (Ser189/207) Antibody #9231 | NIH/3T3 | UV2 (40 mJ/cm2), 30 min recovery time | MKK3/MKK6 Control Cell Extracts #9233 |
| Phospho-MKK3/MKK6 (Ser189/207) (22A8) Rabbit mAb #9236 | NIH/3T3 | UV2 (40 mJ/cm2), 30 min recovery time | MKK3/MKK6 Control Cell Extracts #9233 |
| Phospho-MKK7 (Ser271/Thr275) Antibody #4171 | U937 | Sorbitol (400 mM for 15 min) | |
| Phospho-Mnk1 (Thr197/202) Antibody #2111 | NIH/3T3 | Serum-starve overnight, 20% serum for 20 min | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-MKP1 (Ser359) (125E2) Rabbit mAb #2857 | HeLa | ALLN (50 µM for 5-6 hrs) | |
| Phospho-MLK3 (Thr277/Ser281) Antibody #2811 | Jurkat | Untreated | |
| Phospho-MSK1 (Ser360) Antibody #9594 | Transfected1 | Transfect with construct overexpressing MSK1 | |
| Phospho-MSK1 (Ser376) Antibody #9591 | 293 HEK | UV2 (40 mJ/cm2), 30 min recovery time | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-MSK1 (Thr581) Antibody #9595 | 293 HEK | UV2 (40 mJ/cm2), 30 min recovery time | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-Mst1 (Thr183)/Mst2 (Thr180) Antibody #3681 | WEHI | Staurosporine (1 µM for 30 min - 3 hrs) | |
| Phospho-mTOR (Ser2448) Antibody #2971 | NIH/3T3 | Serum-starve overnight, 20% calf serum for 30 min | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-mTOR (Ser2481) Antibody #2974 | NIH/3T3 | Serum-starve overnight, 20% calf serum for 30 min | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-c-Myc (Thr58/Ser62) Antibody #9401 | A431 | Serum-starve overnight, TPA (200 nM for 30 min) | |
| Phospho-Myosin Light Chain 2 (Thr18/Ser19) Antibody #3674 | C2C12 | Untreated | |
| Phospho-Myosin Light Chain 2 (Ser19) Antibody #3671 | C2C12 | Untreated | |
| Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb #3675 | C2C12 | Untreated | |
| Phospho-Myt1 (Ser83) Antibody #4281 | HeLa | Nocodazole (50 ng/ml for 20 hrs) | |
| Phospho-NF-κB p65 (Ser276) Antibody #3037 | HeLa | TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Acetyl-NF-κB p65 (Lys310) Antibody #3045 | Transfected1 | Cotransfect constructs overexpressing p65 and CBP/p300 | |
| Phospho-NF-κB p65 (Ser468) Antibody #3039 | HeLa | TNF-α (20 ng/ml for 5 min) | NF-κB Control Cell Extracts #9243 |
| Phospho-NF-κB p65 (Ser536) Antibody #3031 | HeLa | TNF-α (20 ng/ml for 5 min) | NF-κB Control Cell Extracts #9243 |
| Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 | HeLa | TNF-α (20 ng/ml for 5 min) | NF-κB Control Cell Extracts #9243 |
| Phospho-NF-κB p65 (Ser536) (7F1) Mouse mAb #3036 | HeLa | TNF-α (20 ng/ml for 5 min) | NF-κB Control Cell Extracts #9243 |
| Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb #4806 | HeLa | TNF-α (20 ng/ml for 5 min) | NF-κB Control Cell Extracts #9243 |
| Phospho-NF-κB2 p100 (Ser866/870) Antibody #4810 | Transfected1 | Cotransfect contructs overexpressing NIK and p100 | |
| Phospho-NMDAR1 (Ser890) Antibody #3381 | Transfected1 | Transfect with construct expressing NMDAR1 and treat with PMA (100 nM for 5 min) | |
| Phospho-NMDAR1 (Ser896) Antibody #3384 | Transfected1 | Transfect with construct expressing NMDAR1 and treat with PMA (100 nM for 5 min) | |
| Phospho-NMDAR1 (Ser897) Antibody #3385 | Transfected1 | Transfect with construct expressing NMDAR1 and treat with Forskolin (30 μM for 20 min) | |
| Cleaved Notch 1 (Val1744) Antibody #2421 | Transfected1 | Transfect cells with construct expressing Notch1 and treat with EDTA (5mM for 15 min) | |
| Phospho-NPM (Thr199) Antibody #3541 | HeLa | Nocodazole (50 ng/ml for 20 hrs) | |
| Phospho-δ-Opioid Receptor (Ser363) Antibody #3641 | Transfected1 | Transfect 293 cells with a construct overexpressing delta-opioid receptor and treat with DPDPE (1μM for 30 min) | |
| Phospho-μ-Opioid Receptor (Ser375) Antibody #3451 | Transfected1 | Transfect 293 cells with mu-opioid receptor and treat with DAMGO (1mM for 5 min) | |
| Phospho-p130 Cas (Tyr165) Antibody #4015 | NIH/3T3 | Untreated | |
| Phospho-p130 Cas (Tyr249) Antibody #4014 | NIH/3T3 | Untreated | |
| Phospho-p130 Cas (Tyr410) Antibody #4011 | NIH/3T3 | Untreated | |
| Phospho-p38 MAP Kinase (Thr180/Tyr182) Antibody #9211 | C6 | Anisomycin (25 μg/ml) for 20 min | p38 MAP Kinase Control Cell Extracts #9213 |
| Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215 | C6 | Anisomycin (25 μg/ml) for 20 min | p38 MAP Kinase Control Cell Extracts #9213 |
| Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb #9216 | C6 | Anisomycin (25 μg/ml) for 20 min | p38 MAP Kinase Control Cell Extracts #9213 |
| Phospho-p40 phox (Thr154) Antibody #4311 | THP1 | Serum-starve overnight, TPA (200 nM for 15 min) | |
| Phospho-p44/42 MAP Kinase (Thr202/Tyr204) Antibody #9101 | HeLa | TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-p44/42 MAPK (Thr202/Tyr204) (197G2) Rabbit mAb #4377 | HeLa | TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-p44/42 MAPK (Thr202/Tyr204) (E10) Mouse mAb #9106 | HeLa | TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-p53 (Ser6) Antibody #9285 | Hek293 | UV2 (75 mJ/cm2), 2 hrs recovery | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-p53 (Ser9) Antibody #9288 | COS | UV2 (75 mJ/cm2), 2 hrs recovery | |
| Phospho-p53 (Thr18) Antibody #2529 | MCF-7 | Doxorubicin (0.5µM for 24 hrs) | |
| Phospho-p53 (Ser15) Antibody #9284 | Hek293 | UV2 (75 mJ/cm2), 2 hrs recovery | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-p53 (Ser15) (16G8) Mouse mAb #9286 | Hek293 | UV2 (75 mJ/cm2), 2 hrs recovery | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-p53 (Ser20) Antibody #9287 | Hek293 | IR (10 Gy), 1 hr recovery | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-p53 (Ser33) Antibody #2526 | HT29 | Nocodazole (50 ng/ml for 24-48 hrs) | |
| Phospho-p53 (Ser37) Antibody #9289 | Hek293 | UV2 (75 mJ/cm2), 2 hrs recovery | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-p53 (Ser46) Antibody #2521 | COS | UV2 (75 mJ/cm2), 2 hrs recovery | |
| Phospho-p53 (Thr81) Antibody #2676 | HeLa | Nocodazole (50 ng/ml for 24-48 hrs) | |
| Phospho-p53 (Ser315) Antibody #2528 | HT29 | Nocodazole (50 ng/ml for 24-48 hrs) | |
| Acetyl-p53 (Lys379) Antibody #2570 | MCF-7 | Doxorubicin (0.5 µM for 24 hrs); 400 nM TSA can be used in conjunction to stabilize activated protein | |
| Acetylated-p53 (Lys382) Antibody #2525 | MCF-7 | Doxorubicin (0.5 µM for 24 hrs);400 nM TSA can be used in conjunction to stabilize activated protein | |
| Phospho-p53 (Ser392) Antibody #9281 | Hek293 | Hydroxyurea (20 mM for 30 hrs) | |
| Phospho-p56Dok-2(Tyr351) Antibody #3911 | Transfected1 | Transfect cells with a construct overexpressing p56Dok-2 | |
| Phospho-p57 Kip2 (Thr310) Antibody #2558 | HeLa | Dexamethasone (50 nM for 16 hrs) | |
| Phospho-p63 (Ser160/162) Antibody #4981 | Me180 | Nocodazole (100 ng/ml for 18 hrs) | |
| Phospho-p63 (Ser395) Antibody #4984 | Me180 | Nocodazole (100 ng/ml for 18 hrs) | |
| Phospho-p63 (Ser455) Antibody #4985 | Me180 | Nocodazole (100 ng/ml for 18 hrs) | |
| Phospho-p70 S6 Kinase (Ser371) Antibody #9208 | NIH/3T3 | Serum-starve overnight, 20% calf serum for 30 min | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-p70 S6 Kinase (Thr389) Antibody #9205 | NIH/3T3 | Serum-starve overnight, 20% calf serum for 30 min | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb #9234 | NIH/3T3 | Serum-starve overnight, 20% calf serum for 30 min | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-p70 S6 Kinase (Thr389) (1A5) Mouse mAb #9206 | NIH/3T3 | Serum-starve overnight, 20% calf serum for 30 min | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-Drosophila p70 S6 Kinase (Thr398) Antibody #9209 | S2 | EGF (30 min at 100 ng/ml) | |
| Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody #9204 | NIH/3T3 | Serum-starve overnight, 20% calf serum for 30 min | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-p73 (Tyr99) Antibody #4665 | HT-1376 | Pervanadate (1mM for 20 min) | |
| Phospho-(Tyr) p85 PI3K Binding Motif Antibody #3821 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-PI3K p85 (Tyr458)/p55 (Tyr199) Antibody #4228 | NIH/3T3/Src | Untreated | |
| Phospho-p90RSK (Thr359/Ser363) Antibody #9344 | HeLa | TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-p90RSK (Ser380) Antibody #9341 | HeLa | TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-p90RSK (Ser380) (9D9) Rabbit mAb #9335 | HeLa | TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-p90RSK (Thr573) Antibody #9346 | HeLa | TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-p95/NBS1 (Ser343) Antibody #3001 | HeLa | UV2 (40 mJ/cm2), 90 min recovery | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-PAK1 (Ser144)/PAK2 (Ser141) Antibody #2606 | Guinea pig neutrophils | fMLP (1 µM for 30 seconds) | |
| Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody #2605 | Guinea pig neutrophils | fMLP (1 µM for 30 seconds) | |
| Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody #2601 | Guinea pig neutrophils | fMLP (1 µM for 30 seconds) | |
| Phospho-PAK2 (Ser20) Antibody #2607 | Guinea pig neutrophils | fMLP (1 µM for 30 seconds) | |
| Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) Antibody #3241 | Guinea pig neutrophils | fMLP (1 µM for 30 seconds) | |
| Phospho-PAR-4 (Thr163) Antibody #2329 | HeLa | Calyculin A (100 nM for 5 min) | |
| Cleaved PARP (Asp214) Antibody (Human Specific) #9541 | HeLa | Serum-starve overnight, (1 µM staurosporine for 3 hrs) | Jurkat Apoptosis Cell Lysates (etoposide) #2043 |
| Cleaved PARP (Asp214) Antibody (Mouse Specific) #9544 | L929 | Serum-starve overnight, (1 µM staurosporine for 3 hrs) | |
| Cleaved PARP (Asp214) Antibody (Rat Specific) #9545 | C6 | Serum-starve overnight, (1 µM staurosporine for 3 hrs) | |
| Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific) #9546 | HeLa | Serum-starve overnight, (1 µM staurosporine for 3 hrs) | Jurkat Apoptosis Cell Lysates (etoposide) #2043 |
| Cleaved PARP (Asp214) (7C9) Mouse mAb (Mouse Specific) #9548 | L929 | Serum-starve overnight, (1 µM staurosporine for 3 hrs) | |
| Phospho-Paxillin (Tyr118) Antibody #2541 | C2C12, NIH/3T3, A431, C6 | Untreated | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-PBK/TOPK (Thr9) Antibody #4941 | HT29 | Nocodazole (50 ng/ml for 20 hrs) | |
| Phospho-PDGF Receptor α (Tyr754) (23B2) Rabbit mAb #2992 | NIH/3T3 | Serum-starve overnight, PDGF-β (100 ng/ml for 5 min) | |
| Phospho-PDGF Receptor β (Tyr740) (32A9) Rabbit mAb #3168 | NIH/3T3 | Serum-starve overnight, PDGF-β (100 ng/ml for 5 min) | |
| Phospho-PDGF Receptor β (Tyr751) Antibody #3161 | NIH/3T3 | Serum-starve overnight, PDGF-β (100 ng/ml for 5 min) | |
| Phospho-PDGF Receptor β (Tyr751) (88H8) Mouse mAb #3166 | NIH/3T3 | Serum-starve overnight, PDGF-β (100 ng/ml for 5 min) | |
| Phospho-PDGF Receptor β (Tyr771) (76D6) Rabbit mAb #3173 | NIH/3T3 | Serum-starve overnight, PDGF-β (100 ng/ml for 5 min) | |
| Phospho-PDGF Receptor β (Tyr1009) (42F9) Rabbit mAb #3124 | NIH/3T3 | Serum-starve overnight, PDGF-β (100 ng/ml for 5 min) | |
| Phospho-PDGF Receptor β (Tyr1021) (6F10) Rabbit mAb #2227 | NIH/3T3 | Serum-starve overnight, PDGF-β (100 ng/ml for 5 min) | |
| Phospho-PDK1 (Ser241) Antibody #3061 | HeLa | Untreated | |
| Phospho-PEA-15 (Ser104) Antibody #2776 | C6 | TPA (200 nM for 1 hr) | |
| Phospho-PERK (Thr980) (16F8) Rabbit mAb #3179 | AR42J | Thapsigargin (1 μM for 20 min) | |
| Phospho-Pim-1 (Tyr218) Antibody #4721 | Jurkat | Untreated | |
| Phospho-Pin1 (Ser16) Antibody #3721 | Ovcar 8 | Forskolin (30 µM for 30 min) | |
| Phospho-PKA C (Thr197) Antibody #4781 | HeLa | Untreated | |
| Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb #9624 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-(Ser/Thr) PKA Substrate Antibody #9621 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-PKC (pan) (βII Ser660) Antibody #9371 | HeLa, NIH/3T3 | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-PKC (pan) (γ Thr514) Antibody #9379 | HeLa, NIH/3T3 | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-PKC (pan) (ζ Thr410) (190D10) Rabbit mAb #2060 | HeLa, NIH/3T3 | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-(Ser) PKC Substrate Antibody #2261 | HeLa | TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-PKCα/β II (Thr638/641) Antibody #9375 | HeLa, NIH/3T3 | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-PKCδ (Tyr311) Antibody #2055 | HeLa, NIH/3T3 | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-PKCδ (Thr505) Antibody #9374 | HeLa, NIH/3T3 | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-PKCδ/θ (Ser643/676) Antibody #9376 | HeLa, NIH/3T3 | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-PKCθ (Thr538) Antibody #9377 | HeLa, NIH/3T3 | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-PKCζ/λ (Thr410/403) Antibody #9378 | HeLa, NIH/3T3 | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-PKD/PKCμ (Ser744/748) Antibody #2054 | HeLa, NIH/3T3 | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-PKD/PKCμ (Ser916) Antibody #2051 | HeLa, NIH/3T3 | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-PKR (Thr451) Antibody #3075 | HeLa, HT29 | IFN-α (1000 U/ml for 18 hrs) | |
| Phospho-PLCβ3 (Ser537) Antibody #2481 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-PLCβ3 (Ser1105) Antibody #2484 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-PLCγ1 (Tyr783) Antibody #2821 | NIH/3T3 | Serum-starve overnight, PDGF (50 ng/ml for 30 min) | |
| Phospho-PLCγ2 (Tyr759) Antibody #3874 | Ramos, Raji | IgM (12µg/ml for 2 min) | |
| Phospho-PLCγ2 (Tyr1217) Antibody #3871 | Ramos, Raji | IgM (12µg/ml for 2 min) | |
| Phospho-PLD1 (Thr147) Antibody #3831 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-PLD1 (Ser561) Antibody #3834 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-PP1α (Thr320) Antibody #2581 | HeLa, COS, NIH/3T3, C6 | Untreated | |
| Phospho-PRK1 (Thr774)/PRK2 (Thr816) Antibody #2611 | HeLa | Untreated | |
| Phospho-Progesterone Receptor (Ser190) Antibody #3171 | T47D | Serum-starve overnight, promegestone (100 nM for 1 hr) | |
| Phospho-PTEN (Ser380) Antibody #9551 | HeLa | Untreated | |
| Phospho-PTEN (Ser380/Thr382/383) Antibody #9554 | HeLa | Untreated | |
| Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb #9549 | HeLa | Untreated | |
| Phospho-PTPα (Tyr789) Antibody #4481 | 293, COS, C6, C2C12 | Untreated | |
| Phospho-Pyk2 (Tyr402) Antibody #3291 | Jurkat | Serum-starve overnight, anti CD3 plus anti CD28 (both 1 µg/ml for 10 min) | |
| Phospho-Rac1/cdc42 (Ser71) Antibody #2461 | A431 | Serum-starve overnight, EGF (100 ng/ml for 10 min) | |
| Phospho-Rad17 (Ser645) Antibody #3421 | COS | UV2 (100 mJ/cm2), 1 hr recovery | |
| Phospho-c-Raf (Ser259) Antibody #9421 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-c-Raf (Ser289/296/301) Antibody #9431 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-c-Raf (Ser296) Antibody #9423 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-c-Raf (Ser338) (56A6) Rabbit mAb #9427 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-Ras-GRF1 (Ser916) Antibody #3321 | Transfected1 | Transfect with construct overexpressing Ras-GRF1 | |
| Phospho-Rb (Ser608) Antibody #2181 | HT-29 | Nocodazole (100 ng/ml for 18 hrs); treatment induces slight phospho-shift | |
| Phospho-Rb (Ser780) Antibody #9307 | HT-29 | Nocodazole (100 ng/ml for 18 hrs); treatment induces slight phospho-shift | |
| Phospho-Rb (Ser795) Antibody #9301 | HT-29 | Nocodazole (100 ng/ml for 18 hrs); treatment induces slight phospho-shift | |
| Phospho-Rb (Ser807/811) Antibody #9308 | HT-29 | Nocodazole (100 ng/ml for 18 hrs); treatment induces slight phospho-shift | |
| Phospho-Ret (Tyr905) Antibody #3221 | TT | Untreated | |
| Phospho-Rev-erbα (Ser55/59) Antibody #2129 | Transfected1 | Transfect with a construct overexpressing Rev-Erbα | |
| Phospho-RIP2 (Ser176) Antibody #4364 | THP-1 | Treat overnight with TPA (50 ng/ml), then culture in fresh medium 48 h to form differentiated macrophages. Induce with LPS (1 ug/ml for 10 min). Dorsch, M. et al. (2006) Cell. Signal. 18, 2223-2229. | |
| Phospho-Rpb1 CTD (Ser2/5) Antibody #4735 | MCF7 | Doxorubicin (0.5 µM for 30 hrs) | |
| Phospho-RSK3 (Thr356/Ser360) Antibody #9348 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-S6 Ribosomal Protein (Ser235/236) Antibody #2211 | NIH/3T3 | Serum-starve overnight, 20% calf serum (30 min) | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb #4856 | NIH/3T3 | Serum-starve overnight, 20% calf serum (30 min) | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-S6 Ribosomal Protein (Ser240/244) Antibody #2215 | NIH/3T3 | Serum-starve overnight, 20% calf serum (30 min) | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-S6 Ribosomal Protein (Ser240/244) (61H9) Rabbit mAb #4838 | NIH/3T3 | Serum-starve overnight, 20% calf serum (30 min) | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-SAPK/JNK (Thr183/Tyr185) Antibody #9251 | 293 HEK | UV2 (40 mJ/cm2), 30 min recovery | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-SAPK/JNK(T183/Y185)(98F2) Rabbit mAb #4671 | 293 HEK | UV2 (40 mJ/cm2), 30 min recovery | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb #9255 | 293 HEK | UV2 (40 mJ/cm2), 30 min recovery | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-SEK1/MKK4 (Ser80) Antibody #9155 | NIH/3T3 | PDGF (100 ng/ml for 5 min) | |
| Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody #9156 | HeLa | UV2 (50 mJ/cm2), 30 min recovery | |
| Phospho-SEK1/MKK4 (Thr261) Antibody #9151 | HeLa | UV2 (50 mJ/cm2), 30 min recovery | |
| Phospho-SGK (Ser78) Antibody #3271 | Transfected1 | Transfect with construct overexpressing SGK | |
| Phospho-Shc (Tyr239/240) Antibody #2434 | HepG2 | Serum-starve overnight, EGF (100 ng/ml for 5 min) | |
| Phospho-Shc (Tyr317) Antibody #2431 | HepG2 | Serum-starve overnight, EGF (100 ng/ml for 5 min) | |
| Phospho-SHIP1 (Tyr1020) Antibody #3941 | Ramos | Anti-human IgM (12 µg/ml for 2 min) | |
| Phospho-SHP-2 (Tyr542) Antibody #3751 | NIH/3T3 | Serum-starve overnight, PDGF (100 ng/ml for 5 min) | |
| Phospho-SirT1 (Ser27) Antibody #2327 | 293 | Untreated | |
| Phospho-SirT1 (Ser47) Antibody #2314 | 293 | Untreated | |
| Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad8 (Ser426/428) Antibody #9511 | NIH/3T3 | Serum-starve overnight, BMP (50 ng/ml for 30 min) | |
| Phospho-Smad2 (Ser245/250/255) Antibody #3104 | NIH/3T3 | Serum-starve overnight, TPA (200 nM for 30 min) | |
| Phospho-Smad2 (Ser465/467) Antibody #3101 | NIH/3T3 | Serum-starve overnight, TGF-β (10 ng/ml for 30 min) | |
| Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108 | NIH/3T3 | Serum-starve overnight, TGF-β (10 ng/ml for 30 min) | |
| Phospho-Smad3 (Ser423/425)/Smad1 (Ser463/465) Antibody #9514 | NIH/3T3 | Serum-starve overnight, TGF-β (10 ng/ml for 30 min) | |
| Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb #9516 | NIH/3T3 | Serum-starve overnight, BMP (50 ng/ml for 30 min) | |
| Phospho-SMC1 (Ser957) Antibody #4801 | HeLa | UV2 (100 mJ/cm2), 1 hr recovery | |
| Phospho-SMC1 (Ser957) (5D11G5) Mouse mAb #4805 | Raw | UV2 (100 mJ/cm2), 1 hr recovery | |
| Phospho-Src (Tyr527) Antibody #2105 | COLO201 | Untreated | |
| Phospho-Src Family (Tyr416) Antibody #2101 | COLO201 | Untreated | |
| Phospho-Src Family (Tyr416) (100F9) Rabbit mAb #2113 | COLO201 | Serum-starve overnight, treat 5 min 20% FBS | |
| Phospho-SRF (Ser103) Antibody #4261 | HeLa | Serum-starve overnight, TPA (15min at 200 nM) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-Stat1 (Tyr701) Antibody #9171 | HeLa | Serum-starve overnight, IFN-α (100 ng/ml for 5 min) | Stat1 Control Cell Extracts #9173 |
| Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167 | HeLa | Serum-starve overnight, IFN-α (100 ng/ml for 5 min) | Stat1 Control Cell Extracts #9173 |
| Phospho-Stat1(Ser727) Antibody #9177 | HeLa | Serum-starve overnight, IFN-γ, 100 ng/ml, 5-10 min | |
| Phospho-Stat2 (Tyr690) Antibody #4441 | HeLa | Serum-starve overnight, IFN-α (100 ng/ml for 15 min) | |
| Acetyl-Stat3 (Lys685) Antibody #2523 | Transfected1 | Cotransfect with contructs expressing Stat3 and CBP (p300/CREB Binding Protein) | |
| Phospho-Stat3 (Tyr705) Antibody #9131 | HeLa | Serum-starve overnight, IFN-α (100 ng/ml for 5 min) | Stat3 Control Cell Extracts #9133 |
| Phospho-Stat3 (Tyr705) (D3A7) Rabbit mAb #9145 | HeLa | Serum-starve overnight, IFN-α (100 ng/ml for 5 min) | Stat3 Control Cell Extracts #9133 |
| Phospho-Stat3 (Ser727) Antibody #9134 | HeLa | Serum-starve overnight, IL-6, 100 ng/ml, 5-10 min | |
| Phospho-Stat3 (Tyr705) (3E2) Mouse mAb #9138 | HeLa | Serum-starve overnight, IFN-α (100 ng/ml for 5 min) | Stat3 Control Cell Extracts #9133 |
| Phospho-Stat3 (Ser727) (6E4) Mouse mAb #9136 | HeLa | Serum-starve overnight, IL-6, (100 ng/ml for 5-10 min) | |
| Phospho-Stat5 (Tyr694) Antibody #9351 | HeLa | Serum-starve overnight, IFN-α (100 ng/ml for 5 min) | Stat5 Control Cell Extracts #9353 |
| Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb #9359 | HeLa | Serum-starve overnight, IFN-α (100 ng/ml for 5 min) | Stat5 Control Cell Extracts #9353 |
| Phospho-Stat5(Tyr694) (14H2) Mouse mAb #9356 | HeLa | Serum-starve overnight, IFN-α (100 ng/ml for 5 min) | Stat5 Control Cell Extracts #9353 |
| Phospho-Stat6 (Tyr641) Antibody #9361 | HeLa | Serum-starve overnight, IL-4 (100 ng/ml for 15 min) | |
| Phospho-Syk (Tyr323) Antibody #2715 | Transfected1 | Transfect with a construct overexpressing Syk | |
| Phospho-Syk (Tyr525/526) Antibody #2711 | Transfected1 | Transfect with a construct overexpressing Syk | |
| Phospho-Synapsin (Ser9) Antibody #2311 | PC-12 | NGF (100 ng/ml for 2 hrs) | |
| Phospho-TAK1 (Thr184) Antibody #4537 | HeLa | 20 ng/ml TNF-α or IL-1β, both with Calyculin A (100 nM) for 10 min | |
| Phospho-TAK1 (Thr184/187) Antibody #4531 | HeLa | 20 ng/ml TNF-α or IL-1β, both with Calyculin A (100 nM) for 10 min | |
| Phospho-TAK1 (Thr184/187) (90C7) Rabbit mAb #4508 | HeLa | 20 ng/ml TNF-α or IL-1β, both with Calyculin A (100 nM) for 10 min | |
| Phospho-TAK1 (Thr187) Antibody #4536 | HeLa | 20 ng/ml TNF-α or IL-1β, both with Calyculin A (100 nM) for 10 min | |
| Phospho-TAK1 (Ser412) Antibody #9339 | K562 | Untreated | |
| Phospho-τ (Ser396) (PHF13) Mouse mAb #9632 | Mouse brain, rat brain | Untreated | |
| Phospho-Threonine Antibody (P-Thr-Polyclonal) #9381 | NIH/3T3 | PDGF-BB (100 ng/ml for 5 min) | |
| Phospho-Threonine (42H4) Mouse mAb #9386 | NIH/3T3 | PDGF-BB (100 ng/ml for 5 min) | |
| Phospho-Threonine-X-Arginine Antibody #2351 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101) #9391 | Jurkat | Nocadazole (1 µg/ml for 12 hrs) | |
| Phospho-Tie2 (Tyr992) Antibody #4221 | Transfected1 | 293 cells transfected with wild-type Tie2 | |
| Phospho-Tie2 (Ser1119) Antibody #4226 | Transfected1 | Sf9 cells overexpressing GST-human Tie2 kinase domain fusion protein | |
| Phospho-TLK1 (Ser695) Antibody #4121 | SKNMC | Serum-starve overnight | |
| Phospho-Topoisomerase IIα (Ser1106) Antibody #2418 | COS, HeLa | Untreated | |
| Phospho-Tpl2 (Ser400) Antibody #4491 | Transfected1 | Transfect with a construct overexpressing Tpl2 | |
| Phospho-TrkA (Tyr490) Antibody #9141 | PC-12 | NGF (100 ng/ml for 2-5 min) | |
| Phospho-Troponin I (Cardiac) (Ser23/24) Antibody #4004 | Primary cardiac myocytes | Isoproterenol | |
| Phospho-Tuberin/TSC2 (Ser939) Antibody #3615 | NIH/3T3 | PDGF (50 ng/mL for 30 min) | |
| Phospho-Tuberin/TSC2 (Ser1254) Antibody #3616 | MCF-7 | Untreated | |
| Phospho-Tuberin/TSC2 (Thr1462) Antibody #3611 | MCF-7 | EGF (10 ng/mL for 30 min) | |
| Phospho-Tuberin/TSC2 (Thr1462) (5B12) Rabbit mAb #3617 | MCF-7 | EGF (10 ng/mL for 30 min) | |
| Phospho-Tuberin/TSC2 (Tyr1571) Antibody #3614 | NIH/3T3 | PDGF (50 ng/mL for 30 min) | |
| Phospho-Tyk2 (Tyr1054/1055) Antibody #9321 | HeLa | Serum-starve overnight, IFN-α (100 ng/ml for 15 min) | |
| Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 | Jurkat | Pervanadate (1 mM for 30 min) | |
| Phospho-Tyrosine Mouse mAb (P-Tyr-102) #9416 | Jurkat | Pervanadate (1 mM for 30 min) | |
| Phospho-Tyrosine Hydroxylase (Ser40) Antibody #2791 | HeLa | TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-VASP (Ser157) Antibody #3111 | A431 | Serum-starve overnight, forskolin (10 µM for 30 min) | |
| Phospho-VASP (Ser239) Antibody #3114 | A431 | Serum-starve overnight, forskolin (10 µM for 30 min) | |
| Phospho-VEGF Receptor 2 (Tyr951) Antibody #2471 | Transfected1 | CKR/PAE cells expressing chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGFR-2 intracellular domains | |
| Phospho-VEGF Receptor 2 (Tyr951) (15D2) Rabbit mAb #4991 | Transfected1 | CKR/PAE cells expressing chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGFR-2 intracellular domains | |
| Phospho-VEGF Receptor 2 (Tyr951) (7H11) Mouse mAb #2476 | Transfected1 | CKR/PAE cells expressing chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGFR-2 intracellular domains | |
| Phospho-VEGF Receptor 2 (Tyr996) Antibody #2474 | Transfected1 | CKR/PAE cells expressing chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGFR-2 intracellular domains | |
| Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb #2478 | HUVEC | Serum-starve overnight, VEGF(100 ng/ml for 5 min) | |
| Phospho-VEGF Receptor 2 (Tyr1212) (11A3) Rabbit mAb #2477 | Transfected1 | CKR/PAE cells expressing chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGFR-2 intracellular domains | |
| Phospho-WNK1 (Thr60) Antibody #4946 | HT29 | Serum-starve overnight, IGF1 (100 ng/ml for 30 min) | |
| Phospho-YAP (Ser127) Antibody #4911 | HeLa | Untreated | |
| Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody #2701 | Jurkat | Serum-starve overnight, H2O2 (2mM for 2 min) | |
| Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb #2717 | Jurkat | Serum-starve overnight, H2O2 (2mM for 2 min) | |
| Phospho-Zap-70 (Tyr493) Antibody #2704 | Jurkat | Serum-starve overnight, H2O2 (2mM for 2 min) | |
