Specific For: BrdU (Bu20a) Mouse mAb #5292
NOTE: Prepare solutions with Milli-Q or equivalently purified water.
NOTE: Cells should be grown, treated, fixed and stained directly in multi-well plates, chamber slides or on coverslips.
BrdU Incorporation: Dilute BrdU in fresh, pre-warmed growth medium to a final concentration of 0.03 mg/mL. Add mixture to cells and incubate at 37°C for 30 minutes.
NOTE: All subsequent incubations should be carried out at room temperature unless otherwise noted in a humid light-tight box or covered dish/plate to prevent drying and fluorochrome fading.
*Recommended Secondary Antibodies:
Anti-Mouse
posted November 2009
revised December 2010