Protocols
NOTE: For the best possible results, Cell Signaling Technology (CST) strongly recommends using our optimized application-specific protocols for each product. These protocols are the result of extensive in-house validation performed at CST and ensure accurate and reproducible results.
NOTE: When available, product specific protocols will be linked from matching product web pages.
ChIP
- SimpleChIP® Chromatin IP Protocol (Agarose Beads) (ChIP Agarose)
- SimpleChIP® Chromatin IP Protocol (Magnetic Beads) (ChIP Magnetic)
- SimpleChIP® Plus Chromatin IP Protocol (Agarose Beads) (ChIP Agarose)
- SimpleChIP® Plus Chromatin IP Protocol (Magnetic Beads) (ChIP Magnetic)
- ChIP Troubleshooting Guide
ELISA
- ELISA-Peptide Assay Protocol (ELISA-P)
- Enzyme Immuno Assay Protocol (EIA)
- HRP Chemiluminescence ELISA-P (HRP Chemiluminescent ELISA-P)
- PathScan® Chemiluminescent ELISA Protocol (Chemiluminescent ELISA)
- PathScan® Sandwich ELISA Antibody Pair Protocol (ELISA-Pair)
- PathScan® Sandwich ELISA Protocol (Sandwich ELISA)
Flow
- Flow Cytometry Protocol (Flow)
- Protocol for Combined Staining of Intracellular Proteins and Cell Surface Markers in Blood (Flow Alternate)
Immunofluorescence
- Immunofluorescence Protocol (IF)
- Immunofluorescence Protocol for Biotin-Conjugated Antibodies (IF Biotin)
- Immunofluorescence Protocol with DOTMAC Permeabilization (IF DOTMAC)
- Immunofluorescence Protocol with Methanol Permeabilization (IF Methanol)
Immunohistochemistry
- Immunohistochemistry Protocol (Frozen) for SignalStain® Boost Detection Reagent (IHC / Frozen)
- Immunohistochemistry Protocol (Paraffin) for SignalStain® Boost Detection Reagent (IHC / Paraffin)
Immunoprecipitation
- Immunoprecipitation of Denatured Proteins (IP / Denatured)
- Immunoprecipitation Protocol / (For Native Protein) (IP / Native Protein)
- Immunoprecipitation Protocol Utilizing Magnetic Separation / (For Analysis By Western Immunoblotting) (IP Magnetic)
- Immobilized Immunoprecipitation Protocol / (For Analysis By Western Immunoblotting) (Immobilized IP)
In-Cell Western
- PhosphoPlus® In-Cell Duet (ICW Compatible) Protocol (In-Cell Duet)
- In-Cell Western Protocol (In-Cell Western)
Western Immunoblot
- Western Immunoblotting Protocol (Western)
- Western Immunoblotting (Fluorescent) Protocol (Fluorescent Western)
- Western Blot Reprobing Protocol (Western / Reprobe)
- KinomeView™ Protocol (Kinomeview Profiling Kit)
Western Troubleshooting:
