Research Protocols

• ChIP
• ELISA
• Flow
• IF
• IHC
• IP
• In-Cell Western
• Western

For the best possible results, Cell Signaling Technology (CST) strongly recommends using our optimized application-specific protocols for each product. These protocols are the result of extensive in-house validation performed at CST and ensure accurate and reproducible results.

Please note: when available, product specific protocols will be linked from matching product web pages.

ChIP

• Enzymatic Chromatin IP (Agarose Beads) Protocol (ChIP Agarose)
• Enzymatic Chromatin IP (Magnetic Beads) Protocol (ChIP Magnetic)

ELISA

• ELISA-Peptide Assay Protocol (ELISA-P)
• PathScan^® Chemiluminescent ELISA Protocol (Chemiluminescent ELISA)
• PathScan^® Sandwich ELISA Antibody Pair Protocol (ELISA-Pair)
• PathScan^® Sandwich ELISA Protocol (Colorimetric) (Sandwich ELISA)

Flow

• Flow Cytometry Protocol (Flow)
• Alternate Protocol for Signaling Analysis in Peripheral Blood Labeled with Surface Markers (Flow Alternate)

Immunofluorescence

• Immunofluorescence Protocol (IF)
• Immunofluorescence Protocol for Biotin-Conjugated Antibodies (IF Biotin)
• Immunofluorescence Protocol with Methanol Permeabilization (IF Methanol)

Immunohistochemistry

• Immunohistochemistry Protocol (Frozen) for SignalStain^® Boost Detection Reagent (IHC / Frozen)
• Immunohistochemistry Protocol (Paraffin) for SignalStain^® Boost Detection Reagent (IHC / Paraffin)

Immunoprecipitation

• Immunoprecipitation of Denatured Proteins (IP / Denatured)
• Immunoprecipitation Protocol / (For Native Protein) (IP / Native Protein)

In-Cell Western

• In-Cell Western Protocol (In-Cell Western)

Western Immunoblot

• Western Immunoblotting Protocol (Western)
• Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In BSA) (Fluorescent Western BSA)
• Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In Milk) (Fluorescent Western Milk)
• Western Blot Reprobing Protocol (Western / Reprobe)
• Western Immunoblotting Troubleshooting Guide